Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
AKAP12/Gravin Antibody (BS-1381R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AKAP12/Gravin Polyclonal Antibody, Unconjugated (bs-1381R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG an... View More
Product Details
BS-1381R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human of human AKAP12, amino acids 701-900.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein (gravin) 12; A kinase (PRKA) anchor protein 12; A-kinase anchor protein 12; A-kinase anchor protein 250 kDa; A-kinase anchor protein, 250kDa; AKAP 250; AKAP-12; gercelin; Germ cell lineage protein gercelin; Gravin; kinase scaffold protein gravin; Myasthenia gravis autoantigen; myasthenia gravis autoantigen gravin; Src-Suppressed C Kinase Substrate; SSeCKS; SSeCKS, Gravin, AKAP250; testis specific gene A12; v-src suppressed transcript 5
Gene Aliases: AI317366; AKAP12; AKAP12A; AKAP12B; AKAP12G; AKAP250; Gag12; Srcs5; SSeCKS; Tsga12
UniProt ID: (Human) Q02952, (Mouse) Q9WTQ5, (Rat) Q5QD51
Entrez Gene ID: (Human) 9590, (Mouse) 83397, (Rat) 83425
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support