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Invitrogen
AKAP6 Polyclonal Antibody
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FIGURE: 1 / 2
AKAP6 Antibody (PA5-103319) in ICC/IF
Immunocytochemistry analysis of AKAP6 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with AKAP6 polyclonal antibody (Product # PA5-103319) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103319
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human AKAP6(Accession Q13023), corresponding to amino acid residues P2232-L2282.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852664
Product Specific Information
Antibody detects endogenous levels of total AKAP6.
Target Information
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is highly expressed in various brain regions and cardiac and skeletal muscle. It is specifically localized to the sarcoplasmic reticulum and nuclear membrane, and is involved in anchoring PKA to the nuclear membrane or sarcoplasmic reticulum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein 6; A-kinase anchor protein 100 kDa; A-kinase anchor protein 6; A-kinase anchoring protein alpha; A-kinase anchoring protein beta; AKAP 100; AKAP-6; anchor protein 6; mAKAP; PRKA6; protein kinase A anchoring protein 6; Protein kinase A-anchoring protein 6
Gene Aliases: ADAP100; ADAP6; AI482140; AKAP100; AKAP6; Akapalpha; Akapbeta; KIAA0311; mAKAP; PRKA; PRKA6
UniProt ID: (Human) Q13023
Entrez Gene ID: (Human) 9472, (Mouse) 238161
positive regulation of protein phosphorylation
regulation of protein kinase A signaling
positive regulation of cell growth
negative regulation of cAMP biosynthetic process
positive regulation of protein phosphatase type 2B activity
cellular protein complex localization
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of NFAT protein import into nucleus
regulation of membrane repolarization
positive regulation of ryanodine-sensitive calcium-release channel activity
positive regulation of cell growth involved in cardiac muscle cell development
positive regulation of calcineurin-NFAT signaling cascade
cellular response to cAMP
cellular response to cytokine stimulus
cellular response to epinephrine stimulus
regulation of cardiac muscle cell contraction
positive regulation of potassium ion transmembrane transport
regulation of relaxation of cardiac muscle
positive regulation of delayed rectifier potassium channel activity
positive regulation of cAMP-dependent protein kinase activity
action potential
protein targeting
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
cAMP-mediated signaling
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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