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Invitrogen
AKAP8 Recombinant Rabbit Monoclonal Antibody (JE63-83)
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FIGURE: 1 / 6
AKAP8 Antibody (MA5-44789) in ICC/IF
Immunocytochemistry analysis of AKAP 95 in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in AKAP 95 Monoclonal antibody (Product # MA5-44789) using a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451; followed by Goat Anti-Rabbit IgG H&L (iFluor™ 488) secondary antibody at a dilution of 1:1,000. Nuclear DNA was labelled in bl... View More
Product Details
MA5-44789
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE63-83
Immunogen
Recombinant protein within human AKAP 95 aa 1-150/692.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931246
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the R subunit (RII) of PKA interacts with specific RII-anchoring proteins. The family of RII-anchoring proteins has been designated A-kinase anchoring proteins (AKAP). AKAP 95, also known as AKAP 8, is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney and skeletal muscle. During mitosis, AKAP 95 is recruited to the chromosomes and plays an essential role in mitotic progression. Characteristic of its family, AKAP 95 participates in PKA signaling through an interaction with the RII regulatory subunit. In addition, AKAP 95 forms a complex with HA95 and HDAC3 and is required for the deacetylation of Histone H3 in mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A kinase (PRKA) anchor protein 8; A kinase anchor protein 8; A-kinase anchor protein 8; A-kinase anchor protein 95 kDa; A-kinase anchor protein, 95kDa; AKAP 8; AKAP 95; AKAP-8; DKFZp586B1222
Gene Aliases: 1200016A02Rik; AA673585; AKAP 95; AKAP-8; AKAP-95; AKAP8; AKAP95; AU015639
UniProt ID: (Human) O43823, (Mouse) Q9DBR0, (Rat) Q63014
Entrez Gene ID: (Human) 10270, (Mouse) 56399, (Rat) 116633
transcription, DNA-templated
regulation of transcription, DNA-templated
mitotic nuclear division
mitotic chromosome condensation
signal transduction
protein transport
positive regulation of histone deacetylation
regulation of histone phosphorylation
cell cycle G2/M phase transition
innate immune response
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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