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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AKAP8L Antibodies

          Bethyl Laboratories

          AKAP8L/HA95 Polyclonal Antibody

          View all (9) AKAP8L antibodies
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          Cite AKAP8L/HA95 Polyclonal Antibody

          • Antibody Testing Data (1)
          AKAP8L/HA95 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          AKAP8L/HA95 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          AKAP8L/HA95 Antibody (A301-365A) in WB

          Detection of human AKAP8L/HA95 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-AKAP8L/HA95 antibody (Product # A301-365A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. AKAP8L/HA95 was also immunoprecipitated by rabbit anti-AKAP8L/HA95 antibody A301-366A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 sec... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AKAP8L/HA95 Antibody in Western Blot (WB)

          Product Details

          A301-365A

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          -

          Immunoprecipitation (IP)

          2-5 µg/mg lysate
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Region between residue 525 and 575 of human A kinase (PRKA) anchor protein 8-like (helicase A-binding protein 95 kDa).
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.20 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          TBS, pH 7.0 to 8.0, with 0.1% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          The recommended shelf life for this product is 1 year from date of receipt.

          Target Information

          Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export (PubMed:10748171, PubMed:11402034). Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA (PubMed:10761695, PubMed:11884601). May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis (PubMed:11034899). May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta (PubMed:12538639). Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8 (PubMed:16980585). May be involved in regulation of pre-mRNA splicing (PubMed:17594903). [UniProt]

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: A kinase (PRKA) anchor protein 8-like; A-kinase anchor protein 8-like; AKAP8-like protein; HA95; HAP95; Helicase A-binding protein 95; helicase A-binding protein 95 kDa; Homologous to AKAP95 protein; neighbor of A-kinase anchoring protein 95; Neighbor of A-kinase-anchoring protein 95; neighbor of AKAP; Neighbor of AKAP95; testis tissue sperm-binding protein Li 90mP

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          Gene Aliases: AKAP8L; HA95; HAP95; HRIHFB2018; NAKAP; NAKAP95

          View more View less

          UniProt ID: (Human) Q9ULX6

          View more View less

          Entrez Gene ID: (Human) 26993

          View more View less

          Function(s)
          DNA binding protein binding lamin binding DEAD/H-box RNA helicase binding histone deacetylase binding poly(A) RNA binding metal ion binding
          Process(es)
          transcription, DNA-templated mRNA processing mitotic chromosome condensation biological_process RNA splicing regulation of mRNA export from nucleus positive regulation of histone deacetylation regulation of histone phosphorylation cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter nuclear envelope disassembly
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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