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AKIRIN2 Antibody (BS-9082R) in IHC (P)
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AKIRIN2 Polyclonal Antibody, Unconjugated (bs-9082R) at 1:400 overnight at 4°C, DAB staining.
Product Details
BS-9082R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human AKIRIN2.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
The highly conserved, nuclear-localized Akirin1 and Akirin2 proteins critically regulate the transcription of NF-kappa-B-dependent genes and are required for defense against Gram-negative bacteria in the immune deficiency and NF-kappa-B pathways. Akirin1 is dispensable in the mouse, and neither knockout mice nor cells derived from them have obvious distinctive phenotypes. In contrast, Akirin2 is required for development in the mouse and knockout of both Akirin homologs in mice show that Akirin2 is required downstream of toll-like receptor (TLR), TNF-a and IL-1-b signaling, and for the production of IL-6. Akirin2 is functionally closer to the single gene in Drosophila, as the homozygous null D. melanogaster Akirin mutants show a similar, mid-to-early embryonic death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 14-3-3 beta; Akirin-2; akirin2; akirin2 {ECO:0000250|UniProtKB:Q53H80}; Fourteen-three-three beta interactant 1; fourteen-three-three beta interactant 1 {ECO:0000312|EMBL:BAE81786.1}; Fourteen-three-three beta interacting-protein 1
Gene Aliases: 2700059D21Rik; AA114675; AA522011; Akirin-2; AKIRIN2; AU019887; C6orf166; dJ486L4.2; FBI1; RGD1307791
UniProt ID: (Human) Q53H80, (Rat) Q25C79, (Mouse) B1AXD8
Entrez Gene ID: (Human) 55122, (Rat) 297968, (Mouse) 433693
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
positive regulation of cell proliferation
negative regulation of gene expression
positive regulation of endopeptidase activity
response to lipopolysaccharide
positive regulation of interleukin-6 production
innate immune response
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
embryo development
immune system process
regulation of transcription, DNA-templated
multicellular organism development
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