Invitrogen

AKR1C1/AKR1C2 Polyclonal Antibody

View all (2) AKR1C1/AKR1C2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-78752

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2745868

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HepG2 whole cell, human A549 whole cell, human HCCP tissue, rat liver tissue, rat kidney tissue, mosue liver tissue, mosue kidney tissue. ICC/IF: HepG2 cell. Flow: HepG2 cell.

Target Information

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 17-alpha-HSD; 17-alpha-hydroxysteroid dehydrogenase; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase); 20-alpha-HSD; 20-alpha-hydroxysteroid dehydrogenase; 3(or 17)-alpha-hydroxysteroid dehydrogenase; 3-alpha-HSD3; 3-alpha-hydroxysteroid dehydrogenase; aldo-keto reductase C; Aldo-keto reductase family 1 member C1; Aldo-keto reductase family 1 member C2; Aldo-keto reductase family 1 member C21; aldo-keto reductase family 1, member C1; aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase); aldo-keto reductase family 1, member C21; aldo-keto reductase family 1, member C6; Chlordecone reductase homolog HAKRC; Chlordecone reductase homolog HAKRD; DD-2; DD/BABP; DD1; Dihydrodiol dehydrogenase 1; dihydrodiol dehydrogenase 1/2; dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase; Dihydrodiol dehydrogenase 2; dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III; dihydrodiol dehydrogenase isoform DD1; Dihydrodiol dehydrogenase/bile acid-binding protein; HBAB; hepatic dihydrodiol dehydrogenase; High-affinity hepatic bile acid-binding protein; indanol dehydrogenase; pseudo-chlordecone reductase; testicular 17,20-desmolase deficiency; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; type 5 17beta-hydroxysteroid dehydrogenase; type II 3-alpha-hydroxysteroid dehydrogenase; type II dihydrodiol dehydrogenase; Type III 3-alpha-hydroxysteroid dehydrogenase

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Gene Aliases: 2-ALPHA-HSD; 20-ALPHA-HSD; AKR1C-pseudo; AKR1C1; AKR1C2; Akr1c21; Akr1c6; BABP; C9; DD; DD-2; DD/BABP; DD1; DD1/DD2; DD2; DDH; DDH1; DDH2; H-37; HAKRC; HAKRD; HBAB; Hsd17b5; MBAB; MCDR2; SRXY8; TDD

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UniProt ID: (Human) Q04828, (Human) P52895, (Rat) Q6AYQ2

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Entrez Gene ID: (Human) 1645, (Human) 1646, (Rat) 307092, (Rat) 291283

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Function(s)
alditol:NADP+ 1-oxidoreductase activity aldo-keto reductase (NADP) activity protein binding oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor phenanthrene 9,10-monooxygenase activity carboxylic acid binding bile acid binding 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity androsterone dehydrogenase (B-specific) activity ketosteroid monooxygenase activity trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity indanol dehydrogenase activity retinal dehydrogenase activity estradiol 17-beta-dehydrogenase activity androsterone dehydrogenase activity testosterone 17-beta-dehydrogenase (NADP+) activity oxidoreductase activity steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process(es)
response to drug response to estrogen daunorubicin metabolic process doxorubicin metabolic process oxidation-reduction process cellular response to jasmonic acid stimulus prostaglandin metabolic process G-protein coupled receptor signaling pathway digestion steroid metabolic process positive regulation of cell proliferation epithelial cell differentiation progesterone metabolic process positive regulation of protein kinase B signaling cellular response to prostaglandin D stimulus retinoid metabolic process xenobiotic metabolic process bile acid metabolic process bile acid and bile salt transport intestinal cholesterol absorption retinal metabolic process cholesterol homeostasis response to organophosphorus protein homooligomerization
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