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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Proteintech

          AKT Monoclonal Antibody (2C5D1)

          268 References
          View all (64) AKT Pan antibodies
          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite AKT Monoclonal Antibody (2C5D1)

          • Antibody Testing Data (23)
          AKT Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          AKT Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 23

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          AKT Antibody (60203-2-IG) in ICC/IF

          Immunofluorescent analysis of HeLa cells using 60203-2-IG (AKT antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AKT Antibody in Immunocytochemistry (ICC/IF)
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Western Blot (WB)
          AKT Antibody in Flow Cytometry (Flow)
          AKT Antibody in Immunoprecipitation (IP)

          Product Details

          60203-2-IG

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000-1:10,000
          View 256 publications 256 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:100-1:400
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          View 5 publications 5 publications

          Flow Cytometry (Flow)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          2C5D1

          Immunogen

          AKT Fusion Protein Ag16695 (1-224 aa encoded by BC000479)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • CoraLite 488
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          This antibody detects all the members of AKT with/without phospho-modification.

          Immunogen sequence: MSDVAIVKE GWLHKRGEYI KTWRPRYFLL KNDGTFIGYK ERPQDVDQRE APLNNFSVAQ CQLMKTERPR PNTFIIRCLQ WTTVIERTFH VETPEEREEW TTAIQTVADG LKKQEEEEMD FRSGSPSDNS GAEEMEVSLA KPKHRVTMNE FEYLKLLGKG TFGKVILVKE KATGRYYAMK ILKKEVIVAK DEVAHTLTEN RVLQNSRHPF LTALKYSFQT HDRLC (1-224 aa encoded by BC000479)

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB; PKB alpha; PKB beta; PKB gamma; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; Proto-oncogene c-Akt; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: 2410016A19Rik; AI851531; AKT; AKT1; AKT2; AKT3; AW554154; CWS6; D930002M15Rik; HIHGHH; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBB; PKBBETA; PKBG; PRKBA; PRKBB; PRKBG; RAC; RAC-ALPHA; RAC-BETA; RAC-gamma; RAC-PK-gamma; STK-2

          View more View less

          UniProt ID: (Human) P31749, (Human) P31751, (Human) Q9Y243, (Mouse) P31750, (Rat) P47196, (Rat) P47197, (Mouse) Q60823, (Rat) Q63484, (Mouse) Q9WUA6

          View more View less

          Entrez Gene ID: (Human) 207, (Human) 208, (Human) 10000, (Mouse) 11651, (Rat) 24185, (Rat) 25233, (Mouse) 11652, (Rat) 29414, (Mouse) 23797

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
          Process(es)
          mitochondrial genome maintenance protein phosphorylation signal transduction peptidyl-serine phosphorylation intracellular signal transduction positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation response to osmotic stress multicellular organism development insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction positive regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of sodium ion transport positive regulation of glucose metabolic process response to muscle activity positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance response to insulin cellular response to insulin stimulus cellular response to hormone stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis intracellular protein transmembrane transport cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus carbohydrate metabolic process glycogen metabolic process transport apoptotic process phosphorylation activation of GTPase activity protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process translation negative regulation of protein kinase activity activation-induced cell death of T cells inflammatory response cytoskeleton organization germ cell development nervous system development aging apoptotic mitochondrial changes response to hormone negative regulation of autophagy positive regulation of fibroblast migration negative regulation of endopeptidase activity regulation of neuron projection development glucose transport protein ubiquitination cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein localization response to fluid shear stress cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of fat cell differentiation negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide response to oxidative stress hyaluronan metabolic process response to growth hormone response to insulin-like growth factor stimulus cellular protein modification process nitric oxide biosynthetic process cellular response to DNA damage stimulus G-protein coupled receptor signaling pathway cell proliferation response to heat negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling peptidyl-threonine phosphorylation spinal cord development cell differentiation platelet activation endocrine pancreas development T cell costimulation positive regulation of endodeoxyribonuclease activity ERBB2 signaling pathway regulation of mRNA stability positive regulation of epidermal growth factor receptor signaling pathway protein autophosphorylation phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity mammary gland epithelial cell differentiation establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand fat cell differentiation regulation of cell cycle arrest
          It has to be done as per old AB suggested Products section.
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