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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          AKT Pan Polyclonal Antibody

          Advanced Verification
          21 Published Figures
          35 References
          View all (64) AKT Pan antibodies

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          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite AKT Pan Polyclonal Antibody

          • Antibody Testing Data (7)
          • Published Figures (21)
          • Advanced Verification (2)
          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
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          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 30

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          AKT Pan Antibody (44-609G) in ICC/IF

          Immunofluorescence analysis of AKT pan was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with AKT pan Rabbit Polyclonal Antibody (Product # 44-609G) at 1:250 dilution in 0.1%BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 30... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
          AKT Pan Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Pan Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Pan Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody
          AKT Pan Antibody

          Product Details

          44-609G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 33 publications 33 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:250
          -

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the carboxy-terminal region of human Akt1.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533692

          Target Information

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: AKT1 kinase; AKT1m; Akt1m protein; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) homolog-2; pan akt; PKB; PKB alpha; PKB beta; PKB gamma; Protein kinase Akt-2; Protein kinase Akt-3; Protein kinase B; Protein kinase B alpha; Protein kinase B beta; Protein kinase B gamma; protein kinase B, beta; protein kinase B-alpha; Proto-oncogene c-Akt; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase alpha; RAC protein kinase beta; RAC-alpha serine/threonine-protein kinase; RAC-beta serine/threonine protein kinase; RAC-beta serine/threonine-protein kinase; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; STK-2; Thymoma viral proto-oncogene; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like protein 1

          View more View less

          Gene Aliases: 2410016A19Rik; AI851531; AKT; AKT1; AKT2; AKT3; AW554154; CWS6; D930002M15Rik; HIHGHH; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBB; PKBBETA; PKBG; PRKBA; PRKBB; PRKBG; RAC; RAC-ALPHA; RAC-BETA; RAC-gamma; RAC-PK-gamma; STK-2

          View more View less

          UniProt ID: (Human) P31749, (Human) P31751, (Human) Q9Y243, (Mouse) P31750, (Mouse) Q60823, (Mouse) Q9WUA6

          View more View less

          Entrez Gene ID: (Human) 207, (Human) 208, (Human) 10000, (Mouse) 11651, (Mouse) 11652, (Mouse) 23797

          View more View less

          Function(s)
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding
          Process(es)
          mitochondrial genome maintenance protein phosphorylation signal transduction peptidyl-serine phosphorylation intracellular signal transduction positive regulation of protein phosphorylation carbohydrate metabolic process glycogen metabolic process glycogen biosynthetic process glucose metabolic process regulation of translation transport apoptotic process multicellular organism development insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction positive regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of sodium ion transport positive regulation of glucose metabolic process phosphorylation positive regulation of cell migration positive regulation of vesicle fusion positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance cellular response to insulin stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis intracellular protein transmembrane transport cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of endothelial cell proliferation regulation of glycogen biosynthetic process translation negative regulation of protein kinase activity activation-induced cell death of T cells inflammatory response cytoskeleton organization germ cell development nervous system development aging apoptotic mitochondrial changes response to hormone negative regulation of autophagy positive regulation of fibroblast migration negative regulation of endopeptidase activity regulation of neuron projection development glucose transport protein ubiquitination cell projection organization protein catabolic process positive regulation of cell growth regulation of cell migration regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation response to food positive regulation of cellular protein metabolic process positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of protein localization response to fluid shear stress cellular response to vascular endothelial growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis anagen positive regulation of apoptotic process positive regulation of blood vessel endothelial cell migration positive regulation of fat cell differentiation negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to growth factor stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis cellular response to peptide cellular protein modification process nitric oxide biosynthetic process cellular response to DNA damage stimulus response to oxidative stress G-protein coupled receptor signaling pathway cell proliferation response to heat negative regulation of gene expression regulation of phosphatidylinositol 3-kinase signaling peptidyl-threonine phosphorylation spinal cord development cell differentiation platelet activation hyaluronan metabolic process endocrine pancreas development T cell costimulation positive regulation of endodeoxyribonuclease activity ERBB2 signaling pathway regulation of mRNA stability positive regulation of epidermal growth factor receptor signaling pathway protein autophosphorylation phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity response to growth hormone mammary gland epithelial cell differentiation establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand response to osmotic stress response to muscle activity response to nutrient levels fat cell differentiation regulation of cell cycle arrest
          It has to be done as per old AB suggested Products section.
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