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AKT2 Monoclonal Antibody (16G11.E8)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

200-501-E71

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

20 µg/mL
-

Flow Cytometry (Flow)

Assay-dependent
-

ELISA (ELISA)

1:2,000-1:10,000
-

Immunoprecipitation (IP)

1:500- 1:2,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

16G11.E8

Immunogen

Anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-AKT2 antibody is directed against human AKT2. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 88% identity to mouse and 90% identity with rat, therefore, cross reactivity is expected.

Target Information

AKT2 is a phosphoinositide-dependent serine/threonine protein kinase that is enriched in insulin responsive tissues and has been implicated in the metabolic actions of the hormone. Akt is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphatidylinositol 3-kinase (PI 3-kinase). Akt2 is amplified and overexpressed in some human cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: murine thymoma viral (v-akt) homolog-2; PKB beta; Protein kinase Akt-2; Protein kinase B beta; putative v-akt murine thymoma viral oncoprotein 2; RAC protein kinase beta; RAC-beta serine/threonine-protein kinase; RAC-PK-beta; v-akt murine thymoma viral oncogene homolog 2

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Gene Aliases: AKT2; HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA

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UniProt ID: (Human) P31751

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Entrez Gene ID: (Human) 208

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Function(s)
protein serine/threonine kinase activity protein kinase C binding protein binding ATP binding
Process(es)
positive regulation of protein phosphorylation glycogen biosynthetic process glucose metabolic process regulation of translation cellular protein modification process apoptotic process response to osmotic stress signal transduction insulin receptor signaling pathway carbohydrate transport positive regulation of signal transduction negative regulation of plasma membrane long-chain fatty acid transport positive regulation of glucose metabolic process response to muscle activity peptidyl-serine phosphorylation regulation of cell migration positive regulation of cell migration positive regulation of vesicle fusion response to nutrient levels positive regulation of fatty acid beta-oxidation peripheral nervous system myelin maintenance cellular response to insulin stimulus negative regulation of RNA splicing positive regulation of peptidyl-serine phosphorylation intracellular signal transduction negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling positive regulation of nitric oxide biosynthetic process fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import positive regulation of positive chemotaxis mammary gland epithelial cell differentiation intracellular protein transmembrane transport regulation of cell cycle arrest cellular response to organic cyclic compound protein localization to plasma membrane positive regulation of protein targeting to membrane activation of GTPase activity positive regulation of cell motility positive regulation of glucose import in response to insulin stimulus
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