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AKT3 Antibody (MA5-55197) in WB
Western Blot analysis of various lysates using AKT3 Recombinant Monoclonal Antibody (Product # MA5-55197) at 1:60,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55197
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2T9H4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 93-154 of human AKT3.
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667442
Product Specific Information
Immunogen Sequence: EEREEWTEAI QAVADRLQRQ EEERMNCSPT SQIDNIGEEE MDASTTHHKR KTMNDFDYLK LL
Target Information
AKT3 (PKB gamma) has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). It is involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, glycogen synthesis and glucose uptake. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AKT3 kinase; DKFZP434N0250; PKB gamma; Protein kinase Akt-3; Protein kinase B gamma; protein kinase B, gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; thymoma viral proto-oncogene 3; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Gene Aliases: AI851531; AKT3; D930002M15Rik; MPPH; MPPH2; Nmf350; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
UniProt ID: (Human) Q9Y243, (Rat) Q63484, (Mouse) Q9WUA6
Entrez Gene ID: (Human) 10000, (Rat) 29414, (Mouse) 23797
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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