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AKT3 (E17K Mutant) Antibody (PA5-78308) in WB
Western Blot using AKT3 (E17K Mutant) Polyclonal Antibody (Product # PA5-78308). Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT3 (E17K Mutant) Polyclonal Antibody (Product # PA5-78308) diluted at 1:1,000.
Product Details
PA5-78308
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding mutant E17K of human AKT3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735442
Product Specific Information
Positive Control: Akt3(E17K)-transfected 293T
Predicted Reactivity: Mouse (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by AKT3 (E17K Mutant Specific) is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PKB gamma; Protein kinase Akt-3; Protein kinase B gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Gene Aliases: AKT3; MPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-gamma; RAC-PK-gamma; STK-2
UniProt ID: (Human) Q9Y243
Entrez Gene ID: (Human) 10000
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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