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Invitrogen
ALDH18A1 Polyclonal Antibody
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FIGURE: 1 / 2
ALDH18A1 Antibody (PA5-114385) in WB
Western blot analysis of ALDH18A1 in the following samples: Lane 1: human HeLA whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Twee... View More
Product Details
PA5-114385
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human P5CS/ALDH18A1 recombinant protein (Position: M1-A224).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2884841
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human MDA-MB-453 whole cell, human Caco-2 whole cell, human HEK293 whole cell, rat heart tissue, mouse skeletal muscle tissue .|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene is a member of the aldehyde dehydrogenase family and encodes a bifunctional ATP- and NADPH-dependent mitochondrial enzyme with both gamma-glutamyl kinase and gamma-glutamyl phosphate reductase activities. The encoded protein catalyzes the reduction of glutamate to delta1-pyrroline-5-carboxylate, a critical step in the de novo biosynthesis of proline, ornithine and arginine. Mutations in this gene lead to hyperammonemia, hypoornithinemia, hypocitrullinemia, hypoargininemia and hypoprolinemia and may be associated with neurodegeneration, cataracts and connective tissue diseases. Alternatively spliced transcript variants, encoding different isoforms, have been described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aldehyde dehydrogenase 18 family, member A1; Aldehyde dehydrogenase family 18 member A1; Delta-1-pyrroline-5-carboxylate synthase; delta-1-pyrroline-5-carboxylate synthetase; delta1-pyrroline-5-carboxlate synthetase; glutamate gamma-semialdehyde synthetase; MGC117316; P5CS; pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase)
Gene Aliases: 2810433K04Rik; ADCL3; AI429789; ALDH18A1; ARCL3A; GSAS; P5CS; PYCS; SPG9A; SPG9B
UniProt ID: (Human) P54886, (Mouse) Q9Z110
Entrez Gene ID: (Human) 5832, (Rat) 361755, (Mouse) 56454
glutamate 5-kinase activity
glutamate-5-semialdehyde dehydrogenase activity
protein binding
ATP binding
delta1-pyrroline-5-carboxylate synthetase activity
identical protein binding
poly(A) RNA binding
nucleotide binding
catalytic activity
kinase activity
oxidoreductase activity
oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
transferase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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