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Invitrogen
ALDH1A1 Monoclonal Antibody (5A11)
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FIGURE: 1 / 5
ALDH1A1 Antibody (MA5-15692) in ICC/IF
Immunofluorescence analysis of ALDH1A1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ALDH1A1 Monoclonal Antibody (5A11) (Product # MA5-15692) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product... View More
Product Details
MA5-15692
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5A11
Immunogen
Purified recombinant fragment of human ALDH1A1 expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11009627
Product Specific Information
MA5-15692 targets ALDH1A1 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.
The MA5-15692 immunogen is purified recombinant fragment of human ALDH1A1 expressed in E. Coli.
.
MA5-15692 detects ALDH1A1 which has a predicted molecular weight of approximately 55kDa.
Target Information
ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-deoxyglucosone dehydrogenase; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1 family, member A1; Aldehyde dehydrogenase 1 soluble; aldehyde dehydrogenase 1, soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic; aldehyde dehydrogenase, liver cytosolic; ALDH class 1; ALDH-E1; ALHDII; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; epididymis secretory sperm binding protein Li53e; MGC2318; RALDH 1; Retinal dehydrogenase 1; retinaldehyde dehydrogenase 1
Gene Aliases: ALDC; ALDH-E1; ALDH1; ALDH11; ALDH1A1; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1
UniProt ID: (Human) P00352
Entrez Gene ID: (Human) 216
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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