Invitrogen

ALDH1A3 Polyclonal Antibody

ALDH1A3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

ALDH1A3 Antibody (PA5-29188) in ICC/IF

Immunofluorescence analysis of ALDH1A3 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ALDH1A3 Polyclonal Antibody (Product # PA5-29188) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034)... View More

ALDH1A3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALDH1A3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

PA5-29188

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALDH1A3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2546664

Product Specific Information

Recommended positive controls: A431, Huh-7, U87-MG.

Predicted reactivity: Mouse (95%), Rat (95%), Xenopus laevis (83%), Chicken (88%), Rhesus Monkey (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ALDH1A3 are aldehyde dehydrogenase isozymes which are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by the ALDH1A3 gene uses retinal as a substrate, either in a free or cellular retinol-binding protein form.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Repurposing Disulfiram for Targeting of Glioblastoma Stem Cells: An In Vitro Study.

Biomolecules

Zirjacks L,Stransky N,Klumpp L,Prause L,Eckert F,Zips D,Schleicher S,Handgretinger R,Huber SM,Ganser K

Published figure using ALDH1A3 polyclonal antibody (Product # PA5-29188) in Western Blot

Thu Oct 21 00:00:00 UTC 2021

ALDH1A3 Segregated Expression and Nucleus-Associated Proteasomal Degradation Are Common Traits of Glioblastoma Stem Cells.

Biomedicines

Fauß J,Sprang B,Leukel P,Sommer C,Nikolova T,Ringel F,Kim EL

PA5-29188 was used in Immunocytochemistry-immunofluorescence to support the association of ALDH1A3 with glioblastoma stem cells and provide evidence for the regulation of ALDH1A3 activities at the level of protein turnover.

Wed Dec 22 00:00:00 UTC 2021

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

PA5-29188 was used in Immunocytochemistry-immunoflourescence to report that PKCλ expression was enriched in basal-like breast cancer, among breast cancer subtypes, and was correlated with ALDH1A3 expression (p = 0.016, χ2-test).

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

High PKCλ expression is required for ALDH1-positive cancer stem cell function and indicates a poor clinical outcome in late-stage breast cancer patients.

PloS one

Nozaki Y,Motomura H,Tamori S,Kimura Y,Onaga C,Kanai S,Ishihara Y,Ozaki A,Hara Y,Harada Y,Mano Y,Sato T,Sato K,Sasaki K,Ishiguro H,Ohno S,Akimoto K

Thu Sep 10 00:00:00 UTC 2020

Serum erythropoietin levels, breast cancer and breast cancer-initiating cells.

Breast cancer research : BCR

Bhat K,Sandler K,Duhachek-Muggy S,Alli C,Cheng F,Moatamed NA,Magyar CE,Du L,Li G,McCloskey S,Vlashi E,Pajonk F

PA5-29188 was used in Immunohistochemistry to conclude that physiologically slow-rising serum erythropoietin levels in response to tumor-related or chemotherapy-induced anemia, as opposed to large doses of recombinant erythropoietin, do not increase the pool of breast cancer-initiating cells.

Wed Jan 30 00:00:00 UTC 2019

Multi-omics profiling reveals key signaling pathways in ovarian cancer controlled by STAT3.

Theranostics

Lu T,Bankhead A,Ljungman M,Neamati N

PA5-29188 was used in Western Blotting to provide a rich, multi-faceted summary of the molecular mechanisms impacted by STAT3 deletion and provide new insight for STAT3's potential as a therapeutic target in ovarian cancer.

Wed Sep 09 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: acetaldehyde dehydrogenase 6; aldehyde dehydrogenase 1 family, member A3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase family 1, subfamily A3; aldehyde dehydrogenase-6; ALDH1A3; ALDHA1A3; RALDH-3; ralDH3; Retinaldehyde dehydrogenase 3

View more View less

Gene Aliases: ALDH1A3; ALDH1A6; ALDH6; MCOP8; RALDH3; V1

View more View less

UniProt ID: (Human) P47895, (Mouse) Q9JHW9, (Rat) Q8K4D8

View more View less

Entrez Gene ID: (Human) 220, (Mouse) 56847, (Rat) 266603

View more View less

Function(s)

retinal dehydrogenase activity aldehyde dehydrogenase (NAD) activity aldehyde dehydrogenase [NAD(P)+] activity protein homodimerization activity thyroid hormone binding NAD+ binding 3-chloroallyl aldehyde dehydrogenase activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

Process(es)

optic cup morphogenesis involved in camera-type eye development retinoic acid biosynthetic process locomotory behavior nucleus accumbens development inner ear morphogenesis retinol metabolic process retinoic acid metabolic process retinal metabolic process positive regulation of apoptotic process embryonic eye morphogenesis neuromuscular process controlling balance oxidation-reduction process righting reflex olfactory pit development face development cellular aldehyde metabolic process metabolic process embryonic camera-type eye development positive regulation of retinoic acid receptor signaling pathway kidney development pituitary gland development response to drug

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ALDH1A3 Polyclonal Antibody

ALDH1A3 Antibody (PA5-29188) in ICC/IF

Product Details

PA5-29188

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!