Proteintech

ALDOB Polyclonal Antibody

ALDOB Antibody in Immunocytochemistry (ICC/IF)

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ALDOB Antibody (18065-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using ALDOB antibody 18065-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ALDOB Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ALDOB Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18065-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ALDOB Fusion Protein Ag12695 (1-316 aa encoded by BC029399)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.41 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MAHRFPALT QEQKKELSEI AQSIVANGKG ILAADESVGT MGNRLQRIKV ENTEENRRQF REILFSVDSS INQSIGGVIL FHETLYQKDS QGKLFRNILK EKGIVVGIKL DQGGAPLAGT NKETTIQGLD GLSERCAQYK KDGVDFGKWR AVLRIADQCP SSLPIQENAN ALARYASICQ QNGLVPIVEP EVIPDGDHDL EHCQYVTEKV LAAVYKALND HHVYLEGTLL KPNMVTAGHA CTKKYTPEQV AMATVTALHR TVPAAVPGIC FLSGGMSEED ATLNLNAINL CPLPKPWKLS FSGVARLQTR RQPRRLL (1-316 aa encoded by BC029399)

Target Information

Fructose-1,6-bisphosphate aldolase is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fructose-1,6-bisphosphatase is a nonenzymatic safety valve that curtails AKT activation to prevent insulin hyperresponsiveness.

Cell metabolism

Gu L,Zhu Y,Watari K,Lee M,Liu J,Perez S,Thai M,Mayfield JE,Zhang B,Cunha E Rocha K,Li F,Kim LC,Jones AC,Wierzbicki IH,Liu X,Newton AC,Kisseleva T,Lee JH,Ying W,Gonzalez DJ,Saltiel AR,Simon MC,Karin M

Tue Jun 06 00:00:00 UTC 2023

RecQ dysfunction contributes to social and depressive-like behavior and affects aldolase activity in mice.

Neurobiology of disease

Hou Y,Park JH,Dan X,Chu X,Yang B,Hussain M,Croteau DL,Bohr VA

Mon May 01 00:00:00 UTC 2023

Hepatic but not Intestinal FBP1 Is Required for Fructose Metabolism and Tolerance.

Endocrinology

Bai Q,Liu Y,Wang CM,Wang JR,Feng Y,Ma X,Yang X,Shi YN,Zhang WJ

Mon Apr 17 00:00:00 UTC 2023

Aldolase B attenuates clear cell renal cell carcinoma progression by inhibiting CtBP2.

Frontiers of medicine

Tan M,Pan Q,Wu Q,Li J,Wang J

Thu Jun 01 00:00:00 UTC 2023

Aldolase B attenuates clear cell renal cell carcinoma progression by inhibiting CtBP2.

Frontiers of medicine

Tan M,Pan Q,Wu Q,Li J,Wang J

Thu Jun 01 00:00:00 UTC 2023

Aldolase B attenuates clear cell renal cell carcinoma progression by inhibiting CtBP2.

Frontiers of medicine

Tan M,Pan Q,Wu Q,Li J,Wang J

Thu Jun 01 00:00:00 UTC 2023

Proteogenomic characterization identifies clinically relevant subgroups of intrahepatic cholangiocarcinoma.

Cancer cell

Dong L,Lu D,Chen R,Lin Y,Zhu H,Zhang Z,Cai S,Cui P,Song G,Rao D,Yi X,Wu Y,Song N,Liu F,Zou Y,Zhang S,Zhang X,Wang X,Qiu S,Zhou J,Wang S,Zhang X,Shi Y,Figeys D,Ding L,Wang P,Zhang B,Rodriguez H,Gao Q,Gao D,Zhou H,Fan J

Mon Jan 10 00:00:00 UTC 2022

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.

Nature communications

Chen C,Zhang Z,Liu C,Wang B,Liu P,Fang S,Yang F,You Y,Li X

Sun Oct 16 00:00:00 UTC 2022

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.

Nature communications

Chen C,Zhang Z,Liu C,Wang B,Liu P,Fang S,Yang F,You Y,Li X

Sun Oct 16 00:00:00 UTC 2022

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.

Nature communications

Chen C,Zhang Z,Liu C,Wang B,Liu P,Fang S,Yang F,You Y,Li X

Sun Oct 16 00:00:00 UTC 2022

ATF4-dependent fructolysis fuels growth of glioblastoma multiforme.

Nature communications

Chen C,Zhang Z,Liu C,Wang B,Liu P,Fang S,Yang F,You Y,Li X

Sun Oct 16 00:00:00 UTC 2022

O-GlcNAc modification of leucyl-tRNA synthetase 1 integrates leucine and glucose availability to regulate mTORC1 and the metabolic fate of leucine.

Nature communications

Kim K,Yoo HC,Kim BG,Kim S,Sung Y,Yoon I,Yu YC,Park SJ,Kim JH,Myung K,Hwang KY,Kim S,Han JM

Wed May 25 00:00:00 UTC 2022

The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPK.

Nature metabolism

Zhang CS,Li M,Wang Y,Li X,Zong Y,Long S,Zhang M,Feng JW,Wei X,Liu YH,Zhang B,Wu J,Zhang C,Lian W,Ma T,Tian X,Qu Q,Yu Y,Xiong J,Liu DT,Wu Z,Zhu M,Xie C,Wu Y,Xu Z,Yang C,Chen J,Huang G,He Q,Huang X,Zhang L,Sun X,Liu Q,Ghafoor A,Gui F,Zheng K,Wang W,Wang ZC,Yu Y,Zhao Q,Lin SY,Wang ZX,Piao HL,Deng X,Lin SC

Sat Oct 01 00:00:00 UTC 2022

Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca²⁺ release from the ER.

iScience

Inoue R,Tsuno T,Togashi Y,Okuyama T,Sato A,Nishiyama K,Kyohara M,Li J,Fukushima S,Kin T,Miyashita D,Shiba Y,Atobe Y,Kiyonari H,Bando K,Shapiro AMJ,Funakoshi K,Kulkarni RN,Terauchi Y,Shirakawa J

Fri Jul 15 00:00:00 UTC 2022

Uncoupling protein 2 and aldolase B impact insulin release by modulating mitochondrial function and Ca²⁺ release from the ER.

iScience

Inoue R,Tsuno T,Togashi Y,Okuyama T,Sato A,Nishiyama K,Kyohara M,Li J,Fukushima S,Kin T,Miyashita D,Shiba Y,Atobe Y,Kiyonari H,Bando K,Shapiro AMJ,Funakoshi K,Kulkarni RN,Terauchi Y,Shirakawa J

Fri Jul 15 00:00:00 UTC 2022

Multi-omics profiling of living human pancreatic islet donors reveals heterogeneous beta cell trajectories towards type 2 diabetes.

Nature metabolism

Wigger L,Barovic M,Brunner AD,Marzetta F,Schöniger E,Mehl F,Kipke N,Friedland D,Burdet F,Kessler C,Lesche M,Thorens B,Bonifacio E,Legido-Quigley C,Barbier Saint Hilaire P,Delerive P,Dahl A,Klose C,Gerl MJ,Simons K,Aust D,Weitz J,Distler M,Schulte AM,Mann M,Ibberson M,Solimena M

Thu Jul 01 00:00:00 UTC 2021

PFKP Activation Ameliorates Foot Process Fusion in Podocytes in Diabetic Kidney Disease.

Frontiers in endocrinology

Zhang Z,Liang W,Luo Q,Hu H,Yang K,Hu J,Chen Z,Zhu J,Feng J,Zhu Z,Chi Q,Ding G

Wed Mar 02 00:00:00 UTC 2022

Loss of hepatic aldolase B activates Akt and promotes hepatocellular carcinogenesis by destabilizing the Aldob/Akt/PP2A protein complex.

PLoS biology

He X,Li M,Yu H,Liu G,Wang N,Yin C,Tu Q,Narla G,Tao Y,Cheng S,Yin H

Tue Dec 01 00:00:00 UTC 2020

Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.

Journal of proteome research

Zhou Y,Qin S,Sun M,Tang L,Yan X,Kim TK,Caballero J,Glusman G,Brunkow ME,Soloski MJ,Rebman AW,Scavarda C,Cooper D,Omenn GS,Moritz RL,Wormser GP,Price ND,Aucott JN,Hood L

Fri Jan 03 00:00:00 UTC 2020

Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.

Molecular therapy : the journal of the American Society of Gene Therapy

Hu J,Chen Z,Bao L,Zhou L,Hou Y,Liu L,Xiong M,Zhang Y,Wang B,Tao Z,Chen K

Wed Jul 08 00:00:00 UTC 2020

Plasma Protein Comparison between Dairy Cows with Inactive Ovaries and Estrus.

Scientific reports

Zhao C,Shu S,Bai Y,Wang D,Xia C,Xu C

Mon Sep 23 00:00:00 UTC 2019

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Laboratory investigation; a journal of technical methods and pathology

Wang J,Wu Q,Qiu J

Sat Jun 01 00:00:00 UTC 2019

Accumulation of fructose 1,6-bisphosphate protects clear cell renal cell carcinoma from oxidative stress.

Laboratory investigation; a journal of technical methods and pathology

Wang J,Wu Q,Qiu J

Sat Jun 01 00:00:00 UTC 2019

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Free radical biology & medicine

Ma Y,Qi Y,Wang L,Zheng Z,Zhang Y,Zheng J

Mon Apr 01 00:00:00 UTC 2019

SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis.

Free radical biology & medicine

Ma Y,Qi Y,Wang L,Zheng Z,Zhang Y,Zheng J

Mon Apr 01 00:00:00 UTC 2019

The Expression of Aldolase B in Islets Is Negatively Associated With Insulin Secretion in Humans.

The Journal of clinical endocrinology and metabolism

Gerst F,Jaghutriz BA,Staiger H,Schulte AM,Lorza-Gil E,Kaiser G,Panse M,Haug S,Heni M,Schütz M,Stadion M,Schürmann A,Marzetta F,Ibberson M,Sipos B,Fend F,Fleming T,Nawroth PP,Königsrainer A,Nadalin S,Wagner S,Peter A,Fritsche A,Richter D,Solimena M,Häring HU,Ullrich S,Wagner R

Sat Dec 01 00:00:00 UTC 2018

The Expression of Aldolase B in Islets Is Negatively Associated With Insulin Secretion in Humans.

The Journal of clinical endocrinology and metabolism

Sat Dec 01 00:00:00 UTC 2018

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Nature

Zhang CS,Hawley SA,Zong Y,Li M,Wang Z,Gray A,Ma T,Cui J,Feng JW,Zhu M,Wu YQ,Li TY,Ye Z,Lin SY,Yin H,Piao HL,Hardie DG,Lin SC

Thu Aug 03 00:00:00 UTC 2017

Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.

Nature

Zhang CS,Hawley SA,Zong Y,Li M,Wang Z,Gray A,Ma T,Cui J,Feng JW,Zhu M,Wu YQ,Li TY,Ye Z,Lin SY,Yin H,Piao HL,Hardie DG,Lin SC

Thu Aug 03 00:00:00 UTC 2017

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

Mon May 01 00:00:00 UTC 2017

CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.

EMBO reports

Zhong X,Tian S,Zhang X,Diao X,Dong F,Yang J,Li Z,Sun L,Wang L,He X,Wu G,Hu X,Wang L,Song L,Zhang H,Pan X,Li A,Gao P

Mon May 01 00:00:00 UTC 2017

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Oncotarget

Zeng HL,Yang Q,Du H,Li H,Shen Y,Liu T,Chen X,Kamal GM,Guan Q,Cheng L,Wang J,Xu F

Fri Nov 24 00:00:00 UTC 2017

Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.

Oncotarget

Zeng HL,Yang Q,Du H,Li H,Shen Y,Liu T,Chen X,Kamal GM,Guan Q,Cheng L,Wang J,Xu F

Fri Nov 24 00:00:00 UTC 2017

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Cell research

Sun L,Song L,Wan Q,Wu G,Li X,Wang Y,Wang J,Liu Z,Zhong X,He X,Shen S,Pan X,Li A,Wang Y,Gao P,Tang H,Zhang H

Wed Apr 01 00:00:00 UTC 2015

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Molecular cancer

Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP

Thu Sep 17 00:00:00 UTC 2015

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Molecular cancer

Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP

Thu Sep 17 00:00:00 UTC 2015

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Molecular cancer

Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP

Thu Sep 17 00:00:00 UTC 2015

Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma.

Molecular cancer

Tao QF,Yuan SX,Yang F,Yang S,Yang Y,Yuan JH,Wang ZG,Xu QG,Lin KY,Cai J,Yu J,Huang WL,Teng XL,Zhou CC,Wang F,Sun SH,Zhou WP

Thu Sep 17 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Aldolase 2; aldolase 2, B isoform; Aldolase B fructose-biphosphate; Aldolase B, fructose-biphosphate; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; Fructose-bisphosphate aldolase B; Liver-type aldolase

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Gene Aliases: ALDB; Aldo-2; ALDO2; ALDOB; BC016435; LIV10

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UniProt ID: (Human) P05062, (Mouse) Q91Y97

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Entrez Gene ID: (Human) 229, (Mouse) 230163, (Rat) 24190

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Function(s)

fructose-bisphosphate aldolase activity protein binding cytoskeletal protein binding phosphatidylcholine binding identical protein binding ATPase binding fructose-1-phosphate aldolase activity fructose binding catalytic activity lyase activity

Process(es)

liver development fructose metabolic process gluconeogenesis glycolytic process NADH oxidation response to fructose response to zinc ion fructose 1,6-bisphosphate metabolic process cellular response to extracellular stimulus positive regulation of ATPase activity cellular response to insulin stimulus response to drug response to starvation response to amino acid response to copper ion response to glucocorticoid response to cAMP canonical glycolysis fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate vacuolar proton-transporting V-type ATPase complex assembly response to interleukin-6 response to peptide hormone response to carbohydrate response to organic cyclic compound

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ALDOB Polyclonal Antibody

ALDOB Antibody (18065-1-AP) in ICC/IF

Product Details

18065-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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