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FIGURE: 1 / 1
ALK Antibody (MA5-55198) in WB
Western Blot analysis of various lysates using ALK Recombinant Monoclonal Antibody (Product # MA5-55198) at 1:2,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
MA5-55198
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
9Z8H9
Immunogen
Recombinant fusion protein containing a sequence within amino acids 1334-1520 of human ALK.
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667443
Product Specific Information
Immunogen Sequence: SNQEVLEFVT SGGRMDPPKN CPGPVYRIMT QCWQHQPEDR PNFAIILERI EYCTQDPDVI NTALPIEYGP LVEEEEKVPV RPKDPEGVPP LLVSQQAKRE EERSPAAPPP LPTTSSGKAA KKPTAAEISV RVPRGPAVEG GHVNMAFSQS NPPSELHKVH GSRNKPTSLW NPTYGSWFTE KPTKKNN
Target Information
Anaplastic Lymphoma Receptor Tyrosine Kinase (ALK) belongs to the insulin receptor superfamily. It is vital for brain development. Mutations, rearrangements, and amplifications in the ALK gene have been found in tumors, including anaplastic large cell lymphoma, neurblastoma, and non-small cell lung cancer. Chromosomal rearrangement is the most common genetic alteration. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; p80; TFG/ALK
Gene Aliases: ALK; CD246; NBLST3; Tcrz
UniProt ID: (Human) Q9UM73, (Mouse) P97793
Entrez Gene ID: (Human) 238, (Mouse) 11682, (Rat) 266802
NF-kappaB-inducing kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
ATP binding
identical protein binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
activation of MAPK activity
protein phosphorylation
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
nervous system development
phosphorylation
peptidyl-tyrosine phosphorylation
protein autophosphorylation
neuron development
cell proliferation
NIK/NF-kappaB signaling
regulation of apoptotic process
positive regulation of NF-kappaB transcription factor activity
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