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ALKBH8 Antibody (PA5-49590) in IHC (P)
Immunohistochemistry analysis of ALKBH8 in formalin-fixed and paraffin-embedded human spleen. Samples were incubated with ALKBH8 polyclonal antibody (Product # PA5-49590) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Details
PA5-49590
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 494-523 amino acids from the C-terminal region of human ALKBH8.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2635044
Target Information
Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain (PubMed:20123966, PubMed:20308323). Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA (PubMed:20123966, PubMed:20308323). Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys)(PubMed:20308323). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly) (PubMed:21285950). Required for normal survival after DNA damage (PubMed:20308323). May inhibit apoptosis and promote cell survival and angiogenesis (PubMed:19293182).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AlkB homologue 8; alkB, alkylation repair homolog 8; Alkylated DNA repair protein alkB homolog 8; Alpha-ketoglutarate-dependent dioxygenase ALKBH8; probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8; tRNA methyltransferase 9 homolog
Gene Aliases: ABH8; ALKBH8; TRM9; TRMT9
UniProt ID: (Human) Q96BT7
Entrez Gene ID: (Human) 91801
tRNA binding
nucleotide binding
iron ion binding
protein binding
ferrous iron binding
zinc ion binding
tRNA (uracil) methyltransferase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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