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ALY Antibody (PA5-106782) in ICC/IF
Immunocytochemistry analysis of ALY in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ALY polyclonal antibody (Product # PA5-106782) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106782
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ALYREF(Accession Q86V81), corresponding to amino acid residues F129-Q179.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854450
Product Specific Information
Antibody detects endogenous levels of total THOC4.
Target Information
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ally of AML-1 and LEF-1; Aly/REF export factor; bZIP enhancing factor; bZIP-enhancing factor BEF; REF1-I; RNA and export factor binding protein 1; RNA and export factor-binding protein 1; Tcra enhancer-binding factor interacting protein 1; THO complex 4; THO complex subunit 4; Tho4; Transcriptional coactivator Aly/REF
Gene Aliases: ALY; ALY/REF; ALYREF; BEF; REF; REF1; Refbp1; THOC4
UniProt ID: (Human) Q86V81, (Mouse) O08583
Entrez Gene ID: (Human) 10189, (Rat) 690585, (Mouse) 21681
regulation of DNA recombination
osteoblast differentiation
mRNA export from nucleus
replication fork processing
positive regulation of DNA-templated transcription, elongation
viral mRNA export from host cell nucleus
mRNA processing
transport
RNA splicing
mRNA transport
mRNA splicing, via spliceosome
termination of RNA polymerase II transcription
RNA export from nucleus
mRNA 3'-end processing
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