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Invitrogen
AMBRA1 Polyclonal Antibody
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Product Details
PA5-72949
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide derived from mouse activating molecule in Beclin 1-regulated/AMBRA1 protein sequence [amino acids range 1175-1250] [UniProt # A2AH22]
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 30% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2718803
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activating molecule in beclin-1-regulated autophagy; activating molecule in Beclin1-regulated autophagy; Activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DDB1 and CUL4 associated factor 3; DDB1- and CUL4-associated factor 3; ischemia related factor NYW-1; WD repeat domain 94
Gene Aliases: 2310079H06Rik; A130023A14; AA474864; AMBRA1; AV021921; D030051N19Rik; DCAF3; KIAA1736; mKIAA1736; Nyw1; WDR94
UniProt ID: (Human) Q9C0C7, (Mouse) A2AH22
Entrez Gene ID: (Human) 55626, (Rat) 59319, (Mouse) 228361
autophagy
negative regulation of cell proliferation
neural tube development
negative regulation of neuron apoptotic process
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of mitophagy
regulation of autophagosome assembly
macroautophagy
cell differentiation
parkin-mediated mitophagy in response to mitochondrial depolarization
mitophagy in response to mitochondrial depolarization
multicellular organism development
nervous system development
embryo development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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