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OriGene
AMBRA1 Polyclonal Antibody
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AMBRA1 Antibody (TA590501) in WB
Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Cat # PS100001, Left lane) or pCMV6-ENTRY AMBRA1 (Cat # RC206255, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and blotted with anti-AMBRA(Cat # TA590501). Positive lysates [LY413575] (100 µg) and [LC413575] (20 µg) can be purchased separately from OriGene.
Product Details
TA590501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
DNA immunization. This antibody was made against a protein fragment from the C Terminus Region of AMBRA1.
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: activating molecule in beclin-1-regulated autophagy; Activating molecule in BECN1-regulated autophagy protein 1; autophagy/beclin-1 regulator 1; DDB1 and CUL4 associated factor 3; DDB1- and CUL4-associated factor 3; WD repeat domain 94
Gene Aliases: AMBRA1; DCAF3; KIAA1736; WDR94
UniProt ID: (Human) Q9C0C7
Entrez Gene ID: (Human) 55626
negative regulation of cell proliferation
macroautophagy
neural tube development
cell differentiation
negative regulation of neuron apoptotic process
positive regulation of phosphatidylinositol 3-kinase activity
parkin-mediated mitophagy in response to mitochondrial depolarization
mitophagy in response to mitochondrial depolarization
positive regulation of mitophagy
regulation of autophagosome assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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