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Invitrogen
AMOTL1 Polyclonal Antibody
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AMOTL1 Antibody (PA5-116800) in WB
Western blot analysis of AMOTL1 in extracts of various cell lines. Samples were incubated with AMOTL1 Polyclonal antibody (Product # PA5-116800) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-116800
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 810-900 of human AMOTL1 (NP_5708991)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901431
Product Specific Information
Positive Samples: HeLa, MCF7, Mouse heart, Mouse lung, Mouse brain, Rat lung, Rat heart
Immunogen sequence: LTSSQLAEEK KEEKTWKGSI GLLLGKEHHE HASAPLLPPP PTSALSSIAS TTAASSAHAK TGSKDSSTQT DKSAELFWPS MASLPSRGRL S
Target Information
The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Angiomotin-like protein 1; JEAP; junction-enriched and -associated protein; junction-enriched and associated protein
Gene Aliases: 2310010G08Rik; 2310067L22Rik; 4932416D09Rik; AMOTL1; JEAP; mFLJ00155
UniProt ID: (Human) Q8IY63, (Mouse) Q9D4H4
Entrez Gene ID: (Human) 154810, (Mouse) 75723, (Rat) 315430
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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