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Invitrogen
AMPK alpha-2 Monoclonal Antibody (206CT5.4.2)
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AMPK alpha-2 Antibody (MA5-37501) in ICC/IF
Immunocytochemistry/Immunofluorescent analysis of AMPK alpha-2 in Hela cells stained with AMPK alpha-2 Monoclonal Antibody (Product # MA5-37501). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AMPK alpha-2 primary antibody (1:25, 1h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400... View More
Product Details
MA5-37501
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
206CT5.4.2
Immunogen
PRKAA2 recombinant protein
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897433
Target Information
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; ACACA kinase; Acetyl-CoA carboxylase kinase; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMPK alpha 2; AMPK subunit alpha-2; AMPK-alpha-2 chain; EC 2.7.11.1; HMGCR kinase; Hydroxymethylglutaryl-CoA reductase kinase; protein kinase, AMP-activated, alpha 2 catalytic subunit
Gene Aliases: AMPK; AMPK2; AMPKa2; PRKAA; PRKAA2
UniProt ID: (Human) P54646
Entrez Gene ID: (Human) 5563
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
AMP-activated protein kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
histone serine kinase activity
metal ion binding
[hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity
[acetyl-CoA carboxylase] kinase activity
transcription, DNA-templated
regulation of transcription, DNA-templated
protein phosphorylation
fatty acid biosynthetic process
cholesterol biosynthetic process
carnitine shuttle
response to stress
cell cycle arrest
signal transduction
lipid biosynthetic process
positive regulation of autophagy
response to muscle activity
Wnt signaling pathway
macroautophagy
positive regulation of macroautophagy
regulation of macroautophagy
cellular response to nutrient levels
negative regulation of TOR signaling
histone-serine phosphorylation
intracellular signal transduction
cellular response to drug
cellular response to glucose starvation
regulation of fatty acid biosynthetic process
glucose homeostasis
regulation of circadian rhythm
negative regulation of apoptotic process
positive regulation of glycolytic process
rhythmic process
fatty acid homeostasis
cellular response to prostaglandin E stimulus
regulation of signal transduction by p53 class mediator
regulation of energy homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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