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Invitrogen
AMPK gamma-1,2,3 Polyclonal Antibody
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AMPK gamma-1,2,3 Antibody (PA5-36314) in IHC (P)
Immunohistochemistry analysis of AMPK gamma-1,2,3 in paraffin-embedded human breast carcinoma tissue (cytoplasm and nuclear staining) and negative control (right, with PBS only). Samples were incubated with AMPK gamma-1,2,3 polyclonal antibody (Product # PA5-36314) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
Product Details
PA5-36314
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 50-100 of human AMPK gamma1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2553469
Product Specific Information
This antibody detects endogenous protein at a molecular weight of 38, 54 and 63 kDa.
Purity is >95% by SDS-PAGE.
Target Information
The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5'-AMP-activated protein kinase subunit gamma-1; 5'-AMP-activated protein kinase subunit gamma-2; 5'-AMP-activated protein kinase subunit gamma-3; 5'-AMP-activated protein kinase, gamma-1 subunit; 5'-AMP-activated protein kinase, gamma-3 subunit; 5-AMP-activated protein kinase, gamma-2 subunit; adenosine monophosphate-activated protein kinase gamma 2-subunit; AMP-activated protein kinase gamma 3 subunit; AMP-activated protein kinase gamma subunit; AMP-activated protein kinase gamma2 subunit; AMP-activated protein kinase, noncatalytic gamma-1 subunit; ampk gamma; AMPK gamma 2; AMPK gamma-1 chain; AMPK gamma-2 chain; AMPK gamma-3 chain; AMPK gamma1; AMPK gamma2; AMPK gamma3; AMPK subunit gamma-1; AMPK subunit gamma-2; AMPK subunit gamma-3; AMPK- gamma1; AMPK- gamma2; AMPK- gamma3; AMPKg; H91620p; Protein kinase AMP-activated gamma; protein kinase, AMP-activated, gamma 1 non-catalytic subunit; protein kinase, AMP-activated, gamma 2 non-catalytic subunit; protein kinase, AMP-activated, gamma 3 non-catalytic subunit; protein kinase, AMP-activated, gamma 3 non-catatlytic subunit
Gene Aliases: 2410051C13Rik; AA571379; AAKG; AAKG2; AI854673; Amkg3; AMPKG; AMPKG3; AMPKg3L; AMPKg3S; BB036179; CMH6; H91620p; Prkaac; PRKAG1; PRKAG2; PRKAG3; Prkga1; WPWS
UniProt ID: (Human) P54619, (Human) Q9UGJ0, (Human) Q9UGI9, (Mouse) O54950, (Rat) P80385, (Mouse) Q91WG5, (Mouse) Q8BGM7
Entrez Gene ID: (Human) 5571, (Human) 51422, (Human) 53632, (Mouse) 19082, (Rat) 25520, (Rat) 373545, (Mouse) 108099, (Rat) 301518, (Mouse) 241113
protein kinase activity
AMP-activated protein kinase activity
cAMP-dependent protein kinase activity
protein binding
ATP binding
cAMP-dependent protein kinase regulator activity
AMP binding
protein kinase binding
ADP binding
cAMP-dependent protein kinase inhibitor activity
phosphorylase kinase regulator activity
protein kinase activator activity
nucleotide binding
glycogen biosynthetic process
protein phosphorylation
glucose transport
glycogen metabolic process
regulation of glycolytic process
negative regulation of protein kinase activity
fatty acid biosynthetic process
ATP biosynthetic process
carnitine shuttle
cell cycle arrest
positive regulation of peptidyl-threonine phosphorylation
sterol biosynthetic process
macroautophagy
regulation of fatty acid metabolic process
activation of protein kinase activity
intracellular signal transduction
regulation of fatty acid biosynthetic process
positive regulation of protein kinase activity
regulation of fatty acid oxidation
regulation of glucose import
negative regulation of protein serine/threonine kinase activity
regulation of signal transduction by p53 class mediator
lipid metabolic process
fatty acid metabolic process
signal transduction
spermatogenesis
positive regulation of gene expression
regulation of catalytic activity
protein heterooligomerization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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