Invitrogen

ANGPTL4 Polyclonal Antibody

ANGPTL4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 15

ANGPTL4 Antibody (40-9800) in IHC (P)

Immunohistochemistry analysis of ANGPTL4 showing staining in the cytoplasm and nucleus of paraffin-embedded mouse liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room te... View More

ANGPTL4 Antibody in Immunohistochemistry (IHC)

ANGPTL4 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

ANGPTL4 Antibody in Western Blot, Flow Cytometry (WB, Flow)

ANGPTL4 Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

40-9800

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Flow Cytometry (Flow)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from an internal region of the human ANGTPL4 protein, which differs from rat and mouse by one and two conservative amino acid replacements, respectively

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533491

Target Information

Angiopoietin-like protein 4 (ANGPTL4), is a secreted protein selectively expressed in adipose tissue, liver, and placenta1-3 that plays a variety of roles in vivo, ranging from adipogenesis to angiogenesis to carcinogenesis. The expression of ANGPTL4 is also under nutritional and hormonal control. ANGPTL4 has been observed to induce a strong pro-angiogenic response independent of vascular endothelial growth factor (VEGF), and its expression has been described in hypoxic human tissues as well as a variety of cancers, including liposarcoma, hepatocellular carcinoma, and conventional renal cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

PPARα and PPARγ activation is associated with pleural mesothelioma invasion but therapeutic inhibition is ineffective.

iScience

Orozco Morales ML,Rinaldi CA,de Jong E,Lansley SM,Gummer JPA,Olasz B,Nambiar S,Hope DE,Casey TH,Lee YCG,Leslie C,Nealon G,Shackleford DM,Powell AK,Grimaldi M,Balaguer P,Zemek RM,Bosco A,Piggott MJ,Vrielink A,Lake RA,Lesterhuis WJ

40-9800 was used in Immunohistochemistry to demonstrate that the in vitro anti-tumor effect of GW6471 is off-target.

Fri Jan 21 00:00:00 UTC 2022

STAT3 induces breast cancer growth via ANGPTL4, MMP13 and STC1 secretion by cancer associated fibroblasts.

Oncogene

Avalle L,Raggi L,Monteleone E,Savino A,Viavattene D,Statello L,Camperi A,Stabile SA,Salemme V,De Marzo N,Marino F,Guglielmi C,Lobascio A,Zanini C,Forni M,Incarnato D,Defilippi P,Oliviero S,Poli V

40-9800 was used in Western Blotting to demonstrate that STAT3 is essential for the pro-tumorigenic functions of murine breast cancer CAFs both in vitro and in vivo, and identify a STAT3 signature significantly enriched for genes encoding for secreted proteins.

Tue Mar 01 00:00:00 UTC 2022

ANGPTL4 exacerbates pancreatitis by augmenting acinar cell injury through upregulation of C5a.

EMBO molecular medicine

Jung KH,Son MK,Yan HH,Fang Z,Kim J,Kim SJ,Park JH,Lee JE,Yoon YC,Seo MS,Han BS,Ko S,Suh YJ,Lim JH,Lee DH,Teo Z,Wee JWK,Tan NS,Hong SS

40-9800 was used in Western Blotting to suggest that targeting ANGPTL4 is a potential strategy for the treatment of pancreatitis.

Fri Aug 07 00:00:00 UTC 2020

ANGPTL4 exacerbates pancreatitis by augmenting acinar cell injury through upregulation of C5a.

EMBO molecular medicine

Jung KH,Son MK,Yan HH,Fang Z,Kim J,Kim SJ,Park JH,Lee JE,Yoon YC,Seo MS,Han BS,Ko S,Suh YJ,Lim JH,Lee DH,Teo Z,Wee JWK,Tan NS,Hong SS

40-9800 was used in Western Blotting to suggest that targeting ANGPTL4 is a potential strategy for the treatment of pancreatitis.

Fri Aug 07 00:00:00 UTC 2020

ANGPTL4 exacerbates pancreatitis by augmenting acinar cell injury through upregulation of C5a.

EMBO molecular medicine

Jung KH,Son MK,Yan HH,Fang Z,Kim J,Kim SJ,Park JH,Lee JE,Yoon YC,Seo MS,Han BS,Ko S,Suh YJ,Lim JH,Lee DH,Teo Z,Wee JWK,Tan NS,Hong SS

40-9800 was used in Western Blotting to suggest that targeting ANGPTL4 is a potential strategy for the treatment of pancreatitis.

Fri Aug 07 00:00:00 UTC 2020

A TAZ-ANGPTL4-NOX2 Axis Regulates Ferroptotic Cell Death and Chemoresistance in Epithelial Ovarian Cancer.

Molecular cancer research : MCR

Yang WH,Huang Z,Wu J,Ding CC,Murphy SK,Chi JT

40-9800 was used in Western Blotting to reveal that TAZ promotes ferroptosis in ovarian cancers by regulating ANGPTL4 and NOX, offering a novel therapeutic potential for ovarian tumors with TAZ activation.

Wed Jan 01 00:00:00 UTC 2020

Circulating progenitor cells and the expression of Cxcl12, Cxcr4 and angiopoietin-like 4 during wound healing in the murine ear.

PloS one

Yellowley CE,Toupadakis CA,Vapniarsky N,Wong A

Published figure using ANGPTL4 polyclonal antibody (Product # 40-9800) in Immunohistochemistry

Fri Mar 06 00:00:00 UTC 2020

Hydrogen peroxide promotes the expression of angiopoietin like 4 in RAW264.7 macrophages via MAPK pathways.

Molecular medicine reports

Liu N,Cui C,Sun Y,Zhang F,Wang S,Su G,Cai X

40-9800 was used in Western Blotting to study the mechanism of action by which angiopoietin like 4 promotes atherosclerosis.

Wed Nov 01 00:00:00 UTC 2017

ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression.

Nature communications

Aryal B,Rotllan N,Araldi E,Ramírez CM,He S,Chousterman BG,Fenn AM,Wanschel A,Madrigal-Matute J,Warrier N,Martín-Ventura JL,Swirski FK,Suárez Y,Fernández-Hernando C

40-9800 was used in immunohistochemistry and western blot elucidate how hematopoietic ANGPTL4 deficiency increases atherogenesis

Wed Jul 27 00:00:00 UTC 2016

ANGPTL4 deficiency in haematopoietic cells promotes monocyte expansion and atherosclerosis progression.

Nature communications

Aryal B,Rotllan N,Araldi E,Ramírez CM,He S,Chousterman BG,Fenn AM,Wanschel A,Madrigal-Matute J,Warrier N,Martín-Ventura JL,Swirski FK,Suárez Y,Fernández-Hernando C

40-9800 was used in immunohistochemistry and western blot elucidate how hematopoietic ANGPTL4 deficiency increases atherogenesis

Wed Jul 27 00:00:00 UTC 2016

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using ANGPTL4 polyclonal antibody (Product # 40-9800) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using ANGPTL4 polyclonal antibody (Product # 40-9800) in Flow Cytometry

Fri Feb 01 00:00:00 UTC 2013

Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.

Journal of lipid research

Singh RK,Fullerton MD,Vine D,Bakovic M

40-9800 was used in western blot to determine the mechanisms of hypertriglyceridemia in Pcyt2 heterozygous mice.

Sat Sep 01 00:00:00 UTC 2012

Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.

Journal of lipid research

Singh RK,Fullerton MD,Vine D,Bakovic M

40-9800 was used in western blot to determine the mechanisms of hypertriglyceridemia in Pcyt2 heterozygous mice.

Sat Sep 01 00:00:00 UTC 2012

Angiopoietin like protein 4 expression is decreased in activated macrophages.

Biochemical and biophysical research communications

Feingold KR,Shigenaga JK,Cross AS,Moser A,Grunfeld C

40-9800 was used in western blot to examine the effect of TLR activation on the expression of macrophage ANGPTL4.

Fri May 11 00:00:00 UTC 2012

The acute phase response stimulates the expression of angiopoietin like protein 4.

Biochemical and biophysical research communications

Lu B,Moser A,Shigenaga JK,Grunfeld C,Feingold KR

40-9800 was used in western blot to investigate factors that regulate ANGPTL3 expression

Fri Jan 22 00:00:00 UTC 2010

Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (ANGPTL4).

PloS one

Aronsson L,Huang Y,Parini P,Korach-André M,Håkansson J,Gustafsson JÅ,Pettersson S,Arulampalam V,Rafter J

40-9800 was used in western blot to explore the mechanism by which Lactobacillus paracasei ssp paracasei F19 alters fat storage

Thu Sep 30 00:00:00 UTC 2010

Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (ANGPTL4).

PloS one

Aronsson L,Huang Y,Parini P,Korach-André M,Håkansson J,Gustafsson JÅ,Pettersson S,Arulampalam V,Rafter J

40-9800 was used in western blot to explore the mechanism by which Lactobacillus paracasei ssp paracasei F19 alters fat storage

Thu Sep 30 00:00:00 UTC 2010

Decreased fat storage by Lactobacillus paracasei is associated with increased levels of angiopoietin-like 4 protein (ANGPTL4).

PloS one

Aronsson L,Huang Y,Parini P,Korach-André M,Håkansson J,Gustafsson JÅ,Pettersson S,Arulampalam V,Rafter J

40-9800 was used in western blot to explore the mechanism by which Lactobacillus paracasei ssp paracasei F19 alters fat storage

Thu Sep 30 00:00:00 UTC 2010

Activating peroxisome proliferator-activated receptor gamma mutant promotes tumor growth in vivo by enhancing angiogenesis.

Cancer research

Tian L,Zhou J,Casimiro MC,Liang B,Ojeifo JO,Wang M,Hyslop T,Wang C,Pestell RG

Tue Dec 15 00:00:00 UTC 2009

Enhanced angiogenesis in obesity and in response to PPARgamma activators through adipocyte VEGF and ANGPTL4 production.

American journal of physiology. Endocrinology and metabolism

Gealekman O,Burkart A,Chouinard M,Nicoloro SM,Straubhaar J,Corvera S

Sat Nov 01 00:00:00 UTC 2008

Enhanced angiogenesis in obesity and in response to PPARgamma activators through adipocyte VEGF and ANGPTL4 production.

American journal of physiology. Endocrinology and metabolism

Gealekman O,Burkart A,Chouinard M,Nicoloro SM,Straubhaar J,Corvera S

Sat Nov 01 00:00:00 UTC 2008

Peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) ligands do not potentiate growth of human cancer cell lines.

Carcinogenesis

Hollingshead HE,Killins RL,Borland MG,Girroir EE,Billin AN,Willson TM,Sharma AK,Amin S,Gonzalez FJ,Peters JM

Sat Dec 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: 425O18-1; angiopoietin-like 4; Angiopoietin-like protein 4; Angiopoietin-related protein 4; ANGPTL2; Fasting-i; Fasting-induced adipose factor; fibrinogen/angiopoietin-related protein; hepatic angiopoietin-related protein; Hepatic fibrinogen/angiopoietin-related protein; HFARP; major histocompatibility complex region NG27; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; PPARG angiopoietin related protein; Secreted protein Bk89

View more View less

Gene Aliases: ANGPTL4; ARP4; Bk89; Farp; FIAF; HARP; HFARP; Ng27; NL2; PGAR; Pgarg; PP1158; PSEC0166; TGQTL; UNQ171; UNQ171/PRO197

View more View less

UniProt ID: (Human) Q9BY76, (Mouse) Q9Z1P8, (Rat) Q6TMA8

View more View less

Entrez Gene ID: (Human) 51129, (Mouse) 57875, (Rat) 362850

View more View less

Function(s)

enzyme inhibitor activity protein binding identical protein binding

Process(es)

angiogenesis response to hypoxia cell differentiation negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of lipoprotein lipase activity protein homooligomerization triglyceride homeostasis negative regulation of endothelial cell apoptotic process cellular response to starvation positive regulation of lipid metabolic process regulation of lipid metabolic process negative regulation of catalytic activity protein oligomerization

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ANGPTL4 Polyclonal Antibody

ANGPTL4 Antibody (40-9800) in IHC (P)

Product Details

40-9800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Neutralization (Neu)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!