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          • Primary Antibodies ›
          • AP1M1 Antibodies

          Invitrogen

          AP1M1 Polyclonal Antibody

          1 Reference
          View all (6) AP1M1 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
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          Cite AP1M1 Polyclonal Antibody

          • Antibody Testing Data (5)
          AP1M1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          AP1M1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          AP1M1 Antibody (PA5-104319) in ICC/IF

          Immunofluorescent analysis of AP1M1 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and AP1M1 polyclonal antibody (Product # PA5-104319) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          AP1M1 Antibody in Immunocytochemistry (ICC/IF)
          AP1M1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP1M1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          AP1M1 Antibody in Western Blot (WB)
          AP1M1 Antibody in Western Blot (WB)

          Product Details

          PA5-104319

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human AP1M1(Accession Q9BXS5), corresponding to amino acid residues I291-I341.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2853629

          Product Specific Information

          Antibody detects endogenous levels of total AP1M1.

          Target Information

          The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: adapter-related protein complex 1 subunit mu-1; adaptor protein complex AP-1 mu-1 subunit; Adaptor protein complex AP-1 subunit mu-1; adaptor-related protein complex 1 mu 1 subunit; adaptor-related protein complex 1 mu-1 subunit; Adaptor-related protein complex 1 subunit mu-1; adaptor-related protein complex 1, mu 1 subunit; adaptor-related protein complex AP-1, mu subunit 1; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; ap1m1 {ECO:0000312|RGD:1307653}; clathrin adaptor protein AP47; clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 mu-1 medium chain 1; clathrin assembly protein complex 1, medium chain; clathrin assembly protein complex AP1, mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; golgi adaptor AP-1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; Mu-adaptin 1; Mu1A-adaptin

          View more View less

          Gene Aliases: [m]1A; AA408894; Adtm1A; AP1M1; AP47; CLAPM2; CLTNM; MU-1A; mu1A

          View more View less

          UniProt ID: (Human) Q9BXS5, (Rat) Q32Q06, (Mouse) P35585

          View more View less

          Entrez Gene ID: (Human) 8907, (Rat) 306332, (Mouse) 11767

          View more View less

          Function(s)
          protein binding
          Process(es)
          intracellular protein transport vesicle-mediated transport melanosome organization endosome to melanosome transport transport protein transport viral process antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of defense response to virus by virus
          It has to be done as per old AB suggested Products section.
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