Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
AP1M1 Antibody (PA5-48177) in ICC/IF
Immunocytochemistry analysis of AP1M1 in U-251 MG cells. Samples were incubated with AP1M1 polyclonal antibody (Product # PA5-48177) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on U-251 MG cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
Product Details
PA5-48177
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human AP1M1.
Conjugate
Form
Liquid
Concentration
0.45 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633635
Product Specific Information
Predicted to react with bovine and rat based on sequence homology.
Target Information
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: adapter-related protein complex 1 subunit mu-1; adaptor protein complex AP-1 mu-1 subunit; Adaptor protein complex AP-1 subunit mu-1; adaptor-related protein complex 1 mu 1 subunit; adaptor-related protein complex 1 mu-1 subunit; Adaptor-related protein complex 1 subunit mu-1; adaptor-related protein complex 1, mu 1 subunit; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; clathrin adaptor protein AP47; clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 mu-1 medium chain 1; clathrin assembly protein complex 1, medium chain; clathrin assembly protein complex AP1, mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; golgi adaptor AP-1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; Mu-adaptin 1; Mu1A-adaptin
Gene Aliases: AP1M1; AP47; CLAPM2; CLTNM; MU-1A
UniProt ID: (Human) Q9BXS5
Entrez Gene ID: (Human) 8907
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support