Invitrogen

AP2 alpha Monoclonal Antibody (3B5)

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1 Published Figure
4 References
View all (18) AP2 alpha antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-872

Applications
Tested Dilution
Publications

Western Blot (WB)

1:50 - 1:200
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-

Immunocytochemistry (ICC/IF)

1:50-1:500
View 3 publications 3 publications

Immunoprecipitation (IP)

1:100 - 1:250
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse

Published species

Human, Mouse, Non-human primate

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

N-terminus of human AP2a.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2199412

Product Specific Information

MA1-872 detects AP-2 from human, mouse, and chicken samples.

MA1-872 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence applications. By Western blot, this antibody detects a 45-50 kDa band representing AP-2. For Western blotting, block with BSA only (not milk).

The MA1-872 immunogen is the N-terminus of human AP2a.

Target Information

TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. TFAP2A is the only AP-2 protein required for early morphogenesis of the lens vesicle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: activating enhancer-binding protein 2 alpha; Activating enhancer-binding protein 2-alpha; Activator protein 2; AP-2; AP-2 transcription factor; AP2-alpha; ERF-1; FLJ51761; OTTHUMP00000214240; OTTHUMP00000214243; transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha); Transcription factor AP-2-alpha

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Gene Aliases: AP-2; Ap-2 (a); AP-2alpha; Ap2; AP2alpha; AP2TF; BOFS; Tcfap2a; TFAP2; TFAP2A

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UniProt ID: (Human) P05549, (Mouse) P34056

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Entrez Gene ID: (Human) 7020, (Chicken) 395982, (Mouse) 21418

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding chromatin binding transcription coactivator activity protein binding transcription factor binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein dimerization activity RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase II transcription corepressor activity transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter kidney development neural tube closure lens morphogenesis in camera-type eye outflow tract morphogenesis keratinocyte development optic vesicle morphogenesis optic cup structural organization transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter sensory perception of sound negative regulation of cell proliferation embryonic pattern specification embryonic body morphogenesis positive regulation of gene expression negative regulation of transcription by competitive promoter binding neural crest cell development anterior neuropore closure trigeminal nerve development oculomotor nerve formation forebrain neuron development positive regulation of cell migration positive regulation of bone mineralization response to lipopolysaccharide embryonic forelimb morphogenesis forelimb morphogenesis negative regulation of epidermal growth factor receptor signaling pathway inner ear morphogenesis negative regulation of apoptotic process negative regulation of neuron apoptotic process positive regulation of neuron apoptotic process skin development regulation of cell differentiation regulation of neuron differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter sympathetic nervous system development embryonic camera-type eye morphogenesis embryonic cranial skeleton morphogenesis skeletal system morphogenesis epidermis morphogenesis palate development lens induction in camera-type eye face morphogenesis bone morphogenesis eyelid development in camera-type eye cornea development in camera-type eye positive regulation of tooth mineralization cellular response to iron ion basement membrane organization negative regulation of reactive oxygen species metabolic process response to water deprivation retina layer formation Schwann cell development
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