Proteintech

AP2B1 Polyclonal Antibody

AP2B1 Antibody in Immunocytochemistry (ICC/IF)

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AP2B1 Antibody (15690-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 15690-1-AP) (AP2B1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

AP2B1 Antibody in Immunoprecipitation (IP)

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Product Details

15690-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

AP2B1 Fusion Protein Ag8248 (598-951 aa encoded by BC006201)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GSTDAGDSP VGTTTATNLE QPQVIPSQGD LLGDLLNLDL GPPVNVPQVS SMQMGAVDLL GGGLDSLLGS DLGGGIGGSP AVGQSFIPSS VPATFAPSPT PAVVSSGLND LFELSTGIGM APGGYVAPKA VWLPAVKAKG LEISGTFTHR QGHIYMEMNF TNKALQHMTD FAIQFNKNSF GVIPSTPLAI HTPLMPNQSI DVSLPLNTLG PVMKMEPLNN LQVAVKNNID VFYFSCLIPL NVLFVEDGKM ERQVFLATWK DIPNENELQF QIKECHLNAD TVSSKLQNNN VYTIAKRNVE GQDMLYQSLK LTNGIWILAE LRIQPGNPNY TLSLKCRAPE VSQYIYQVYD SILKN (598-951 aa encoded by BC006201)

Target Information

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Shc and EGF receptor. AP2 is composed of four subunits, two separate ~100 kDa gene products with similar domain structures (alpha and beta adaptin) and a ~50 and ~17 kDa subunit. There are two alpha-adaptin genes, alpha A and alpha C which have a tissue specific pattern of expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Syncytin-mediated open-ended membrane tubular connections facilitate the intercellular transfer of cargos including Cas9 protein.

eLife

Zhang C,Schekman R

Fri Mar 10 00:00:00 UTC 2023

Regulators of proteostasis are translationally repressed in fibroblasts from patients with sporadic and LRRK2-G2019S Parkinson's disease.

NPJ Parkinson's disease

Flinkman D,Hong Y,Gnjatovic J,Deshpande P,Ortutay Z,Peltonen S,Kaasinen V,James P,Coffey E

Mon Feb 06 00:00:00 UTC 2023

Digital color-coded molecular barcoding reveals dysregulation of common FUS and FMRP targets in soma and neurites of ALS mutant motoneurons.

Cell death discovery

Garone MG,Salerno D,Rosa A

Thu Jan 26 00:00:00 UTC 2023

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.

Science China. Life sciences

Li G,Su B,Fu P,Bai Y,Ding G,Li D,Wang J,Yang G,Chu B

Tue Feb 01 00:00:00 UTC 2022

NPC1-regulated dynamic of clathrin-coated pits is essential for viral entry.

Science China. Life sciences

Li G,Su B,Fu P,Bai Y,Ding G,Li D,Wang J,Yang G,Chu B

Tue Feb 01 00:00:00 UTC 2022

Pseudorabies Virus Inhibits Expression of Liver X Receptors to Assist Viral Infection.

Viruses

Wang Y,Li GL,Qi YL,Li LY,Wang LF,Wang CR,Niu XR,Liu TX,Wang J,Yang GY,Zeng L,Chu BB

Thu Mar 03 00:00:00 UTC 2022

The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry.

Journal of virology

Wang G,Jiang L,Wang J,Zhang J,Kong F,Li Q,Yan Y,Huang S,Zhao Y,Liang L,Li J,Sun N,Hu Y,Shi W,Deng G,Chen P,Liu L,Zeng X,Tian G,Bu Z,Chen H,Li C

Mon Jan 06 00:00:00 UTC 2020

Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.

Journal of neuropathology and experimental neurology

Katsumata Y,Fardo DW,Bachstetter AD,Artiushin SC,Wang WX,Wei A,Brzezinski LJ,Nelson BG,Huang Q,Abner EL,Anderson S,Patel I,Shaw BC,Price DA,Niedowicz DM,Wilcock DW,Jicha GA,Neltner JH,Van Eldik LJ,Estus S,Nelson PT

Wed Jan 01 00:00:00 UTC 2020

GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway.

Journal of experimental & clinical cancer research : CR

Zhang XX,Ni B,Li Q,Hu LP,Jiang SH,Li RK,Tian GA,Zhu LL,Li J,Zhang XL,Zhang YL,Yang XM,Yang Q,Wang YH,Zhu CC,Zhang ZG

Wed May 22 00:00:00 UTC 2019

Trans-Golgi protein p230/golgin-245 is involved in phagophore formation.

Biochemical and biophysical research communications

Sohda M,Misumi Y,Ogata S,Sakisaka S,Hirose S,Ikehara Y,Oda K

Fri Jan 02 00:00:00 UTC 2015

Trans-Golgi protein p230/golgin-245 is involved in phagophore formation.

Biochemical and biophysical research communications

Sohda M,Misumi Y,Ogata S,Sakisaka S,Hirose S,Ikehara Y,Oda K

Fri Jan 02 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: adapter-related protein complex 2 beta subunit; adapter-related protein complex 2 subunit beta; adaptin, beta 2 (beta); Adaptor protein complex AP-2 subunit beta; adaptor related protein complex 2, beta 1 subunit; Adaptor-related protein complex 2 subunit beta; adaptor-related protein complex 2, beta 1 subunit; AP-2 complex subunit beta; AP105B; beta adaptin; Beta-2-adaptin; Beta-adaptin; beta2-adaptin; Clathrin assembly protein complex 2 beta large chain; clathrin-associated/assembly/adaptor; clathrin-associated/assembly/adaptor protein, large, beta 1; Plasma membrane adaptor HA2/AP2 adaptin beta subunit; testicular tissue protein Li 22

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Gene Aliases: 1300012O03Rik; ADTB2; AI788979; AP105B; AP2-BETA; AP2B1; CLAPB1

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UniProt ID: (Human) P63010, (Rat) P62944, (Mouse) Q9DBG3

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Entrez Gene ID: (Human) 163, (Rat) 140670, (Mouse) 71770

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Function(s)

signal sequence binding protein binding protein transporter activity clathrin binding protein complex binding clathrin adaptor activity

Process(es)

intracellular protein transport endocytosis clathrin coat assembly transport protein transport vesicle-mediated transport ventricular septum development microtubule-based movement antigen processing and presentation of exogenous peptide antigen via MHC class II aorta development negative regulation of epidermal growth factor receptor signaling pathway ephrin receptor signaling pathway regulation of defense response to virus by virus Wnt signaling pathway, planar cell polarity pathway coronary vasculature development clathrin-mediated endocytosis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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AP2B1 Polyclonal Antibody

AP2B1 Antibody (15690-1-AP) in ICC/IF

Product Details

15690-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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