Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
AP3M1 Antibody (PA5-87877) in WB
Western blot analysis of AP3M1 in extracts of various cell lines. Samples were incubated with AP3M1 Polyclonal antibody (Product # PA5-87877) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-87877
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 219-418 of human AP3M1 (NP_0362271)
Conjugate
Form
Liquid
Concentration
1.78 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804474
Product Specific Information
Immunogen sequence: SLSFMNPRLL DDVSFHPCIR FKRWESERVL SFIPPDGNFR LISYRVSSQN LVAIPVYVKH SISFKENSSC GRFDITIGPK QNMGKTIEGI TVTVHMPKVV LNMNLTPTQG SYTFDPVTKV LTWDVGKITP QKLPSLKGLV NLQSGAPKPE ENPSLNIQFK IQQLAISGLK VNRLDMYGEK YKPFKGVKYV TKAGKFQVRT; Positive Samples: 22Rv1, Mouse liver, Mouse kidney, Rat spinal cord, Rat liver; Cellular Location: Cytoplasmic side, Cytoplasmic vesicle membrane, Golgi apparatus, Peripheral membrane protein
Target Information
The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: adapter-related protein complex 3 mu-1 subunit; adapter-related protein complex 3 subunit mu-1; Adaptor-related protein complex 3 subunit mu-1; adaptor-related protein complex AP-3 mu 1 subunit; adaptor-related protein complex AP-3, mu 1 subunit; AP-3 adapter complex mu3A subunit; AP-3 adaptor complex mu3A subunit; AP-3 complex subunit mu-1; ap3; clathrin adaptor complex AP3, mu-3A subunit; clathrin adaptor protein mu3A; clathrin assembly protein assembly protein complex 1 medium chain homolog 1; clathrin assembly protein assembly protein complex 3 mu-1 medium chain; clathrin coat assembly protein AP47 homolog 1; clathrin coat-associated protein AP47 homolog 1; clathrin-associated adaptor protein homolog (p47A); golgi adaptor AP-1 47 kDa protein homolog 1; HA1 47 kDa subunit homolog 1; Mu-adaptin 3A; Mu3A-adaptin
Gene Aliases: 1200013D09Rik; AP3M1; C78982; P47a; R75378
UniProt ID: (Human) Q9Y2T2, (Mouse) Q9JKC8
Entrez Gene ID: (Human) 26985, (Rat) 171126, (Mouse) 55946
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support