Invitrogen

APH1 Polyclonal Antibody

4 Published Figures

11 References

View all (8) APH1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (4)

APH1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

APH1 Antibody (PA1-2010) in ICC/IF

Immunofluorescent analysis of APH-1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APH-1 Rabbit Polyclonal Antibody (Product # PA1-2010) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

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Product Details

PA1-2010

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of human APH-1aL/CT.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.69 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.005% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2227105

Product Specific Information

PA1-2010 detects APH-1 in human and mouse samples.

PA1-2010 has been successfully used in immunofluorescent, immunoprecipitation, and Western blot procedures. By Western blot, this antibody detects a ~28 kDa protein representing APH-1 in mouse brain and kidney extracts.

The PA1-2010 immunogen is a Synthetic peptide, KLH conjugated, corresponding to residues C (245) R R Q E D S R V M V Y S A L R I P P E D (265) of the C-Terminal domain of the long isoform of APH-1a. This polyclonal antibody has been referred to as, O2C2 in the reference listed above.

Target Information

APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gossypetin ameliorates 5xFAD spatial learning and memory through enhanced phagocytosis against Aβ.

Alzheimer's research & therapy

Jo KW,Lee D,Cha DG,Oh E,Choi YH,Kim S,Park ES,Kim JK,Kim KT

PA1-2010 was used in Western Blotting to study how gossypetin showed protective effects against AD by enhancing microglial Aβ phagocytosis.

Fri Oct 21 00:00:00 UTC 2022

Physical and functional interaction between the α- and γ-secretases: A new model of regulated intramembrane proteolysis.

The Journal of cell biology

Chen AC,Kim S,Shepardson N,Patel S,Hong S,Selkoe DJ

PA1-2010 was used in western blot to identify and characterize a multiprotease complex containing both alpha- and gamma-secretase

Mon Dec 21 00:00:00 UTC 2015

Presenilin transmembrane domain 8 conserved AXXXAXXXG motifs are required for the activity of the γ-secretase complex.

The Journal of biological chemistry

Marinangeli C,Tasiaux B,Opsomer R,Hage S,Sodero AO,Dewachter I,Octave JN,Smith SO,Constantinescu SN,Kienlen-Campard P

PA1-2010 was used in western blot to investigate presenilin assembly and catalytic activity

Fri Mar 13 00:00:00 UTC 2015

Partial loss of presenilin impairs age-dependent neuronal survival in the cerebral cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Watanabe H,Iqbal M,Zheng J,Wines-Samuelson M,Shen J

Published figure using APH1 polyclonal antibody (Product # PA1-2010) in Western Blot

Wed Nov 26 00:00:00 UTC 2014

Familial frontotemporal dementia-associated presenilin-1 c.548G>T mutation causes decreased mRNA expression and reduced presenilin function in knock-in mice.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Watanabe H,Xia D,Kanekiyo T,Kelleher RJ,Shen J

PA1-2010 was used in western blot to study the effects of a familial frontotemporal dementia-associated presenilin-1 c.548G>T mutation in knock-in mice

Wed Apr 11 00:00:00 UTC 2012

Inhibitors of γ-secretase stabilize the complex and differentially affect processing of amyloid precursor protein and other substrates.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Barthet G,Shioi J,Shao Z,Ren Y,Georgakopoulos A,Robakis NK

PA1-2010 was used in western blot to study the effect of gamma-secretase inhibitors on amyloid precursor protein

Thu Sep 01 00:00:00 UTC 2011

Calsenilin regulates presenilin 1/γ-secretase-mediated N-cadherin ε-cleavage and β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Jang C,Choi JK,Na YJ,Jang B,Wasco W,Buxbaum JD,Kim YS,Choi EK

PA1-2010 was used in western blot to identify the role of Calsenilin in N-cadherin cleavage in neuroblastomas

Thu Dec 01 00:00:00 UTC 2011

Caspases-2 and -8 are involved in the presenilin1/gamma-secretase-dependent cleavage of amyloid precursor protein after the induction of apoptosis.

Journal of neuroscience research

Chae SS,Yoo CB,Jo C,Yun SM,Jo SA,Koh YH

PA1-2010 was used in western blot to investigate the involvement of caspase 2 and 8 in the presenilin/gamma-secretase activation and Abeta production in apoptosis

Thu Jul 01 00:00:00 UTC 2010

PI3K inhibition causes the accumulation of ubiquitinated presenilin 1 without affecting the proteasome activity.

Biochemical and biophysical research communications

Aoyagi N,Uemura K,Kuzuya A,Kihara T,Kawamata J,Shimohama S,Kinoshita A,Takahashi R

PA1-2010 was used in western blot to study the role of PI3K in ubiquitination and proteasome activity

Fri Jan 08 00:00:00 UTC 2010

Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria.

The American journal of pathology

Area-Gomez E,de Groof AJ,Boldogh I,Bird TD,Gibson GE,Koehler CM,Yu WH,Duff KE,Yaffe MP,Pon LA,Schon EA

PA1-2010 was used in western blot to study the expression and localization of presenilins in endoplasmic reticulum membranes

Sun Nov 01 00:00:00 UTC 2009

APH-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of presenilin.nicastrin complexes.

The Journal of biological chemistry

Gu Y,Chen F,Sanjo N,Kawarai T,Hasegawa H,Duthie M,Li W,Ruan X,Luthra A,Mount HT,Tandon A,Fraser PE,St George-Hyslop P

PA1-2010 was used in western blot to investigate the role of APH-1 during the formation of presenilin and nicastrin complexes.

Fri Feb 28 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: anterior pharynx defective 1 homolog A; aph-1 homolog A, gamma secretase subunit; APH-1a; Aph-1alpha; APH1A gamma secretase subunit; Gamma-secretase subunit APH-1A; Presenilin-stabilization factor; RP4-790G17.3

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Gene Aliases: 6530402N02Rik; APH-1; APH-1A; APH1A; CGI-78; PSF; UNQ579/PRO1141

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UniProt ID: (Human) Q96BI3, (Mouse) Q8BVF7

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Entrez Gene ID: (Human) 51107, (Mouse) 226548

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Function(s)

endopeptidase activity protein binding peptidase activity

Process(es)

metanephros development proteolysis membrane protein ectodomain proteolysis Notch signaling pathway Notch receptor processing protein processing membrane protein intracellular domain proteolysis amyloid precursor protein catabolic process positive regulation of catalytic activity positive regulation of apoptotic process ephrin receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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APH1 Polyclonal Antibody

APH1 Antibody (PA1-2010) in ICC/IF

Product Details

PA1-2010

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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