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APOBEC3A Antibody (PA5-142329) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of APOBEC3A in A549 cells using APOBEC3A Polyclonal Antibody (Product # PA5-142329). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA5-142329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-TSNFNNGIGRHKTY (aa 19-32).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932980
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:8,000.
Target Information
PHO1 a member of the cytidine deaminase gene family. The PHO1 gene is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. This gene encodes a protein that lacks the zinc binding activity and may be an expressed pseudogene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A3A; apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3A; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A; DNA dC->dU-editing enzyme APOBEC-3A; PHO-1; pho1; phorbolin 1; Phorbolin-1; Phorbolin1; probable DNA dC->dU-editing enzyme APOBEC-3A
Gene Aliases: A3A; APOBEC3A; ARP3; bK150C2.1; PHRBN
UniProt ID: (Human) P31941
Entrez Gene ID: (Human) 200315
cytidine deamination
negative regulation of transposition
clearance of foreign intracellular DNA by conversion of DNA cytidine to uridine
negative regulation of viral genome replication
innate immune response
defense response to virus
DNA cytosine deamination
cellular response to xenobiotic stimulus
DNA demethylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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