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Invitrogen
APPL1 Monoclonal Antibody (5G11)
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FIGURE: 1 / 6
APPL1 Antibody (MA5-49222) in IHC (P)
Immunohistochemistry analysis of APPL1 in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with APPL1 Monoclonal antibody (Product # MA5-49222) using a dilution of 2 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was u... View More
Product Details
MA5-49222
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
5G11
Immunogen
E.coli-derived human APPL/APPL1 recombinant protein (Position: K91-R668).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074880
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human THP-1 whole cell, human PC-3 whole cell, human Hela whole cell, rat brain tissue, mouse brain tissue. IHC: human rectal cancer tissue, human breast cancer tissue, human appendicitis tissue. Flow: U-937 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signaling and insulin signaling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein APPL like; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; AKT2 interactor; DCC-interacting protein 13-alpha; DIP13 alpha; Dip13-alpha; signaling adaptor protein DIP13alpha
Gene Aliases: 2900057D21Rik; 7330406P05Rik; AI585782; APPL; APPL1; AW209077; BB022931; C88264; DIP13; DIP13A; DIP13alpha; KIAA1428; MODY14; RGD1309388
UniProt ID: (Human) Q9UKG1, (Mouse) Q8K3H0
Entrez Gene ID: (Human) 26060, (Rat) 290537, (Mouse) 72993
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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