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ARAP1 Antibody (MA1-19728) in ICC/IF
Immunocytochemistry detection of colocalization of ARAP1 with DR4 at the plasma membrane and in early endosomes. ARAP1-transfected NCTC cells were treated with TRAIL for 0´, 30´ or 60´ and fixed with methanol. DR4 was detected by rabbit polyclonal (red) and ARAP1 with ARAP1-2 mouse monoclonal antibody (green) (Product # MA1-19728). Arrowheads show ARAP1-DR4 colocalization at the plasma membrane and intracellular membranes, whereas arrows show their colocalization in early endosomes.
Product Details
MA1-19728
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
ARAP1-2
Immunogen
His6-tagged protein corresponding to amino acids 1190-1450 of human ARAP1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2227584
Product Specific Information
This antibody reacts with C-terminal part of ARAP1 (intracellular epitope), a 160 kDa adaptor protein.
Target Information
ARAP1 (angiotensin II type 1 receptor-associated protein) is an adaptor protein with ArfGAP and RhoGAP activities, containing five PH domains and ankyrin repeat. This adaptor seems to serve as a link between phosphoinositide- Arf-, and Rho-mediated cell signaling. ARAP1 supports the plasma membrane recycling of angiotensin II type 1 receptor (AT1) and is important also for cell-specific trafficking of pro-death receptor TRAIL-R1 (DR4) to the plasma membrane, thus promoting the TRAIL-induced apoptosis in certain cell types. ARAP1 also affects cell spreading.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1; ARF-GAP, RHO-GAP, ankyrin repeat, and pleckstrin homology domains-containing protein 1; centaurin, delta 2; Centaurin-delta-2; Cnt-d2
Gene Aliases: ARAP1; CENTD2; KIAA0782
UniProt ID: (Human) Q96P48
Entrez Gene ID: (Human) 116985
positive regulation of receptor recycling
signal transduction
regulation of cell shape
actin filament reorganization involved in cell cycle
positive regulation of GTPase activity
positive regulation of epidermal growth factor receptor signaling pathway
regulation of small GTPase mediated signal transduction
regulation of cellular component movement
positive regulation of filopodium assembly
negative regulation of stress fiber assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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