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ARCH Antibody (MA5-24536) in WB
Western blot analysis of mouse ZBTB8OS/ARCH was performed by loading whole-cell lysates of mouse embryonic fibroblasts (each of the three lanes was equally loaded with 10000-15000 cell equivalents) in reducing sample buffer onto a 20-well NuPAGE™ 4-12% Bis-Tris Midi Protein Gels (Product # WG1402A). Proteins were transferred to PVDF membrane with tank transfer. Membrane was blocked in 5% non-fat milk/TBST. Mouse ZBTB8OS/ARCH was detected at approximately 26 kDa using a ZBTB8OS/ARCH monoclonal antibody (Product # MA5-24536) at a dilution of... View More
Product Details
MA5-24536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
1G4
Immunogen
ZBTB8OS (AAH58843.1, 1 a.a. ~ 136 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate
Form
Liquid
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2633508
Product Specific Information
Peptide Sequence: MAQEEEDVRD YNLTEEQKAI KAKYPPVNRK YEYLDHTADV QLHAWGDTLE EAFEQCAMAM FGYMTDTGTV EPLQTVEVET QGDDLQSLLF HFLDEWLYKF SADEFFIPRV GRRIFIVQAP SGNRSQSNNI FSNAGL
Target Information
Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB. Together with DDX1, acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: archease (ARCH); archease-like protein; Protein archease; Protein ZBTB8OS; zinc finger and BTB domain containing 8 opposite strand; zinc finger and BTB domain containing 8 opposite strand, pseudogene 1; Zinc finger and BTB domain-containing opposite strand protein 8
Gene Aliases: ARCH; ARCH2; RGD1562249; ZBTB8OS; Zbtb8os-ps1
UniProt ID: (Human) Q8IWT0
Entrez Gene ID: (Human) 339487, (Rat) 297885
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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