Invitrogen

ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

ARF1/ARF3/ARF5/ARF6 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

ARF1/ARF3/ARF5/ARF6 Antibody (MA3-060) in ICC/IF

Immunofluorescent analysis of ADP-Ribosylation Factor using ADP-Ribosylation Factor Monoclonal Antibody (1D9) (Product # MA3-060) shows staining in Hela Cells. ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody re... View More

ARF1/ARF3/ARF5/ARF6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1/ARF3/ARF5/ARF6 Antibody in Western Blot (WB)

ARF1/ARF3/ARF5/ARF6 Antibody in Flow Cytometry (Flow)

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Product Details

MA3-060

Applications

Tested Dilution

Publications

Western Blot (WB)

1:3000

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

1/20

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Bovine, Hamster, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1D9

Immunogen

Recombinant human ARF1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2274419

Product Specific Information

MA3-060 detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4 from human, mouse and rat tissues.

MA3-060 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a single ~21 kDa protein representing Arf in rat pancreas extract. Immunofluorescence staining of Arf in rat pancreas with MA3-060 results in staining of the cytoplasmic face of the trans-Golgi membranes. MA3-060 cannot be used to neutralize Arf in solution.

The MA3-060 antigen is recombinant human Arf1.

Target Information

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The ARF GAP ELMOD2 acts with different GTPases to regulate centrosomal microtubule nucleation and cytokinesis.

Molecular biology of the cell

Turn RE,East MP,Prekeris R,Kahn RA

MA3-060 was used in Immunocytochemistry to highlight key aspects of signaling by ARF family GAPs that contribute to previously underappreciated sources of complexity, including GAPs acting from multiple sites in cells, working with multiple GTPases, and contributing to the spatial and temporal control of regulatory GTPases by serving as both GAPs and effectors.

Sat Aug 15 00:00:00 UTC 2020

NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization.

Nature communications

Yamanaka T,Tosaki A,Kurosawa M,Matsumoto G,Koike M,Uchiyama Y,Maity SN,Shimogori T,Hattori N,Nukina N

MA3-060 was used in Western Blotting to suggest that nuclear transcription factor-Y (NF-Y) is involved in a unique regulation mechanism of ER organisation in mature neurons.

Tue Feb 25 00:00:00 UTC 2014

ARF1 is directly involved in dynamin-independent endocytosis.

Nature cell biology

Kumari S,Mayor S

MA3-060 was used in western blot to investigate the important role of ARF1 in endocytosis

Tue Jan 01 00:00:00 UTC 2008

Arf GTPase-activating protein ASAP1 interacts with Rab11 effector FIP3 and regulates pericentrosomal localization of transferrin receptor-positive recycling endosome.

Molecular biology of the cell

Inoue H,Ha VL,Prekeris R,Randazzo PA

MA3-060 was used in immunocytochemistry and western blot to report that the BAR domain of ASAP1 is a protein binding site for FIP3

Wed Oct 01 00:00:00 UTC 2008

Activation of cellular Arf GTPases by poliovirus protein 3CD correlates with virus replication.

Journal of virology

Belov GA,Habbersett C,Franco D,Ehrenfeld E

MA3-060 was used in western blot to study the role of cellular Arf GTPases during poliovirus replication

Sat Sep 01 00:00:00 UTC 2007

PI4P promotes the recruitment of the GGA adaptor proteins to the trans-Golgi network and regulates their recognition of the ubiquitin sorting signal.

Molecular biology of the cell

Wang J,Sun HQ,Macia E,Kirchhausen T,Watson H,Bonifacino JS,Yin HL

MA3-060 was used in immunocytochemistry to study the roles of phosphatidylinositol 4 phosphate in cargo recognition at the trans-Golgi network.

Sun Jul 01 00:00:00 UTC 2007

Arf6 plays an early role in platelet activation by collagen and convulxin.

Blood

Choi W,Karim ZA,Whiteheart SW

MA3-060 was used in western blot to study the role of Arf6 in platelet activation by collagen and convulxin

Sat Apr 15 00:00:00 UTC 2006

Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake.

Proceedings of the National Academy of Sciences of the United States of America

Shen X,Xu KF,Fan Q,Pacheco-Rodriguez G,Moss J,Vaughan M

MA3-060 was used in ELISA to investigate the role of the BIG2 in Tfn uptake and TfnR recycling.

Tue Feb 21 00:00:00 UTC 2006

Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake.

Proceedings of the National Academy of Sciences of the United States of America

Shen X,Xu KF,Fan Q,Pacheco-Rodriguez G,Moss J,Vaughan M

MA3-060 was used in ELISA to investigate the role of the BIG2 in Tfn uptake and TfnR recycling.

Tue Feb 21 00:00:00 UTC 2006

Poliovirus proteins induce membrane association of GTPase ADP-ribosylation factor.

Journal of virology

Belov GA,Fogg MH,Ehrenfeld E

MA3-060 was used in western blot to investigate the role of ARF in poliovirus RNA replication

Wed Jun 01 00:00:00 UTC 2005

Requirement for Arf6 in breast cancer invasive activities.

Proceedings of the National Academy of Sciences of the United States of America

Hashimoto S,Onodera Y,Hashimoto A,Tanaka M,Hamaguchi M,Yamada A,Sabe H

MA3-060 was used in western blot to investigate the role of ADP-ribosylation factor 6 (Arf6) in breast cancer invasive activities

Tue Apr 27 00:00:00 UTC 2004

Inhibition of membrane tubule formation and trafficking by isotetrandrine, an antagonist of G-protein-regulated phospholipase A2 enzymes.

Molecular biology of the cell

Chan D,Strang M,Judson B,Brown WJ

MA3-060 was used in immunocytochemistry to investigate the role of G-protein-regulated PLA2 enzyme in membrane tubule formation and trafficking

Thu Apr 01 00:00:00 UTC 2004

Islet cell autoantigen of 69 kDa is an arfaptin-related protein associated with the Golgi complex of insulinoma INS-1 cells.

The Journal of biological chemistry

Spitzenberger F,Pietropaolo S,Verkade P,Habermann B,Lacas-Gervais S,Mziaut H,Pietropaolo M,Solimena M

MA3-060 was used in immunoprecipitation to demonstrate the role of ICA69 in membrane trafficking at the Golgi complex and immature secretory granules in neurosecretory cells.

Fri Jul 11 00:00:00 UTC 2003

Regulation of Golgi structure and function by ARF-like protein 1 (Arl1).

Journal of cell science

Lu L,Horstmann H,Ng C,Hong W

MA3-060 was used in immunocytochemistry to demonstrate the role of ARF-like protein 1 Arl1 in Golgi structure and function by ARF-like protein 1.

Sat Dec 01 00:00:00 UTC 2001

An ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 is involved in subcellular localization of paxillin and actin cytoskeletal organization.

Molecular biology of the cell

Mazaki Y,Hashimoto S,Okawa K,Tsubouchi A,Nakamura K,Yagi R,Yano H,Kondo A,Iwamatsu A,Mizoguchi A,Sabe H

MA3-060 was used in western blot to investigate the role of ADP-ribosylation factor GTPase-activating protein Git2-short/KIAA0148 in paxillin subcellular localization and cytoskeletal organization

Thu Mar 01 00:00:00 UTC 2001

A role for calcium in stabilizing transport vesicle coats.

The Journal of biological chemistry

Ahluwalia JP,Topp JD,Weirather K,Zimmerman M,Stamnes M

MA3-060 was used in western blot to study the role of calcium in transport vesicle coats stabilization.

Fri Sep 07 00:00:00 UTC 2001

GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.

The Journal of cell biology

Dell'Angelica EC,Puertollano R,Mullins C,Aguilar RC,Vargas JD,Hartnell LM,Bonifacino JS

MA3-060 was used in western blot to demonstrate that GGAs are components of coats involved in protein trafficking at the TGN

Mon Apr 03 00:00:00 UTC 2000

ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes.

The Journal of cell biology

Ooi CE,Dell'Angelica EC,Bonifacino JS

MA3-060 was used in western blot to investigate the role for ADP-ribosylation factor 1 in the membrane recruitment of AP-3 adaptor complex .

Mon Jul 27 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: ADP-ribosylation factor 1; ADP-ribosylation factor 2; ADP-ribosylation factor 3; ADP-ribosylation factor 4; ADP-ribosylation factor 4-like protein; ADP-ribosylation factor 5; ADP-ribosylation factor 6; ARF; Liver regeneration-related protein LRRG202; pan ARF; small GTP binding protein

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Gene Aliases: 5430400P17Rik; AA407803; Ac1-253; AI788669; AI854770; ARF1; ARF2; ARF3; ARF4; ARF5; ARF6; AW496366; I79_003112

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UniProt ID: (Human) P84077, (Human) P61204, (Human) P18085, (Human) P84085, (Human) P62330, (Mouse) P84078, (Rat) P84079, (Rat) P84082, (Mouse) Q8BSL7, (Mouse) P61205, (Rat) P61206, (Rat) P61751, (Mouse) P61750, (Mouse) P84084, (Rat) P84083, (Mouse) P62331, (Rat) P62332

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Function(s)

magnesium ion binding GTPase activity receptor signaling protein activity protein binding GTP binding GDP binding poly(A) RNA binding phospholipase D activator activity ARF guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding thioesterase binding protein N-terminus binding nucleotide binding protein homodimerization activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ARF1/ARF3/ARF5/ARF6 Monoclonal Antibody (1D9)

ARF1/ARF3/ARF5/ARF6 Antibody (MA3-060) in ICC/IF

Product Details

MA3-060

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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