Proteintech

ARF1 Polyclonal Antibody

View all (23) ARF1 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (15)

ARF1 Antibody in Immunocytochemistry (ICC/IF)

ARF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

ARF1 Antibody (10790-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ARF1 Antibody in Immunocytochemistry (ICC/IF)

ARF1 Antibody in Immunocytochemistry (ICC/IF)

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

ARF1 Antibody in Western Blot (WB)

Product Image Gallery

ARF1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ARF1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 10790-1-AP (ARF1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ARF1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ARF1 Antibody in Western Blot (WB) — rat brain tissue were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ARF1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ARF1 Antibody in Western Blot (WB) — COS-7 cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ARF1 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 10790-1-AP (ARF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ARF1 Antibody in Western Blot (WB) — WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HEK-293 cells.

ARF1 Antibody in Western Blot (WB) — WB result of ARF1 antibody (10790-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARF1 transfected HeLa cells.

Product Details

10790-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ARF1 Fusion Protein Ag1244 (1-181 aa encoded by BC009247)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can bind ARFs for the close sequences.

Immunogen sequence: MGNIFANLF KGLFGKKEMR ILMVGLDAAG KTTILYKLKL GEIVTTIPTI GFNVETVEYK NISFTVWDVG GQDKIRPLWR HYFQNTQGLI FVVDSNDRER VNEAREELMR MLAEDELRDA VLLVFANKQD LPNAMNAAEI TDKLGLHSLR HRNWYIQATC ATSGDGLYEG LDWLSNQLRN QK (1-181 aa encoded by BC009247)

Target Information

ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HPV is a cargo for the COPI sorting complex during virus entry.

Science advances

Harwood MC,Woo TT,Takeo Y,DiMaio D,Tsai B

Fri Jan 20 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Genetics of mirror movements identifies a multifunctional complex required for Netrin-1 guidance and lateralization of motor control.

Science advances

Schlienger S,Yam PT,Balekoglu N,Ducuing H,Michaud JF,Makihara S,Kramer DK,Chen B,Fasano A,Berardelli A,Hamdan FF,Rouleau GA,Srour M,Charron F

Fri May 12 00:00:00 UTC 2023

Targeting ARF1-IQGAP1 interaction to suppress colorectal cancer metastasis and vemurafenib resistance.

Journal of advanced research

Hu HF,Gao GB,He X,Li YY,Li YJ,Li B,Pan Y,Wang Y,He QY

Fri Sep 01 00:00:00 UTC 2023

Targeting ARF1-IQGAP1 interaction to suppress colorectal cancer metastasis and vemurafenib resistance.

Journal of advanced research

Hu HF,Gao GB,He X,Li YY,Li YJ,Li B,Pan Y,Wang Y,He QY

Fri Sep 01 00:00:00 UTC 2023

Pathogenic variants in TSC2 might cause premature ovarian insufficiency through activated mTOR induced hyperactivation of primordial follicles.

Fertility and sterility

Xu B,Li Z,Li S,Ke H,Zhang Q,Qin Y,Guo T

Thu Dec 01 00:00:00 UTC 2022

Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.

Communications biology

Yue X,Tiwari N,Zhu L,Ngo HDT,Lim JM,Gim B,Jing S,Wang Y,Qian Y,Lee I

Tue Dec 07 00:00:00 UTC 2021

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

BMC biology

Yue X,Qian Y,Zhu L,Gim B,Bao M,Jia J,Jing S,Wang Y,Tan C,Bottanelli F,Ziltener P,Choi S,Hao P,Lee I

Tue Sep 07 00:00:00 UTC 2021

Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Theranostics

Hu HF,Xu WW,Li YJ,He Y,Zhang WX,Liao L,Zhang QH,Han L,Yin XF,Zhao XX,Pan YL,Li B,He QY

Mon Aug 16 00:00:00 UTC 2021

ARF GTPases control phenotypic switching of vascular smooth muscle cells through the regulation of actin function and actin dependent gene expression.

Cellular signalling

Charles R,Bourmoum M,Claing A

Fri Jun 01 00:00:00 UTC 2018

ARF GTPases control phenotypic switching of vascular smooth muscle cells through the regulation of actin function and actin dependent gene expression.

Cellular signalling

Charles R,Bourmoum M,Claing A

Fri Jun 01 00:00:00 UTC 2018

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

Cell host & microbe

Miller CN,Smith EP,Cundiff JA,Knodler LA,Bailey Blackburn J,Lupashin V,Celli J

Wed Sep 13 00:00:00 UTC 2017

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

Cell host & microbe

Miller CN,Smith EP,Cundiff JA,Knodler LA,Bailey Blackburn J,Lupashin V,Celli J

Wed Sep 13 00:00:00 UTC 2017

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Scientific reports

Lei X,Xiao X,Zhang Z,Ma Y,Qi J,Wu C,Xiao Y,Zhou Z,He B,Wang J

Fri Mar 17 00:00:00 UTC 2017

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Scientific reports

Lei X,Xiao X,Zhang Z,Ma Y,Qi J,Wu C,Xiao Y,Zhou Z,He B,Wang J

Fri Mar 17 00:00:00 UTC 2017

ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.

Oncotarget

Schlienger S,Campbell S,Pasquin S,Gaboury L,Claing A

Tue Mar 29 00:00:00 UTC 2016

ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.

Oncotarget

Schlienger S,Campbell S,Pasquin S,Gaboury L,Claing A

Tue Mar 29 00:00:00 UTC 2016

Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.

The EMBO journal

Lee S,Uchida Y,Wang J,Matsudaira T,Nakagawa T,Kishimoto T,Mukai K,Inaba T,Kobayashi T,Molday RS,Taguchi T,Arai H

Wed Mar 04 00:00:00 UTC 2015

Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.

The EMBO journal

Lee S,Uchida Y,Wang J,Matsudaira T,Nakagawa T,Kishimoto T,Mukai K,Inaba T,Kobayashi T,Molday RS,Taguchi T,Arai H

Wed Mar 04 00:00:00 UTC 2015

The small GTPase ADP-Ribosylation Factor 1 mediates the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors.

Cancer biology & therapy

Haines E,Schlienger S,Claing A

Mon Sep 12 00:00:00 UTC 2016

The small GTPase ADP-Ribosylation Factor 1 mediates the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors.

Cancer biology & therapy

Haines E,Schlienger S,Claing A

Mon Sep 12 00:00:00 UTC 2016

ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion.

Molecular biology of the cell

Schlienger S,Campbell S,Claing A

Wed Jan 01 00:00:00 UTC 2014

ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion.

Molecular biology of the cell

Schlienger S,Campbell S,Claing A

Wed Jan 01 00:00:00 UTC 2014

The adaptor proteins p66Shc and Grb2 regulate the activation of the GTPases ARF1 and ARF6 in invasive breast cancer cells.

The Journal of biological chemistry

Haines E,Saucier C,Claing A

Fri Feb 28 00:00:00 UTC 2014

The adaptor proteins p66Shc and Grb2 regulate the activation of the GTPases ARF1 and ARF6 in invasive breast cancer cells.

The Journal of biological chemistry

Haines E,Saucier C,Claing A

Fri Feb 28 00:00:00 UTC 2014

ARF1 controls Rac1 signaling to regulate migration of MDA-MB-231 invasive breast cancer cells.

Cellular signalling

Lewis-Saravalli S,Campbell S,Claing A

Sun Sep 01 00:00:00 UTC 2013

ARF1 controls Rac1 signaling to regulate migration of MDA-MB-231 invasive breast cancer cells.

Cellular signalling

Lewis-Saravalli S,Campbell S,Claing A

Sun Sep 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: ADP-ribosylation factor 1

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Gene Aliases: ARF1

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UniProt ID: (Human) P84077, (Mouse) P84078, (Rat) P84079

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Entrez Gene ID: (Human) 375, (Mouse) 11840, (Rat) 64310

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magnesium ion binding GTPase activity receptor signaling protein activity protein binding GTP binding GDP binding poly(A) RNA binding phospholipase D activator activity nucleotide binding protein homodimerization activity

regulation of receptor internalization phosphatidylinositol biosynthetic process cellular copper ion homeostasis post-Golgi vesicle-mediated transport actin filament organization small GTPase mediated signal transduction protein transport antigen processing and presentation of exogenous peptide antigen via MHC class II regulation of Arp2/3 complex-mediated actin nucleation very-low-density lipoprotein particle assembly positive regulation of catalytic activity positive regulation of endocytosis positive regulation of calcium ion-dependent exocytosis regulation of defense response to virus by virus positive regulation of protein secretion Golgi to transport vesicle transport long term synaptic depression positive regulation of dendritic spine development synaptic vesicle budding dendritic spine organization lysosomal membrane organization positive regulation of sodium ion transmembrane transport positive regulation of late endosome to lysosome transport positive regulation of ER to Golgi vesicle-mediated transport regulation of phospholipid metabolic process transport vesicle-mediated transport

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