Proteintech

ARFIP2 Polyclonal Antibody

View all (9) Arfaptin 2 antibodies

Questions & Answers

Questions & Answers

Antibody Testing Data (12)

ARFIP2 Antibody in Immunocytochemistry (ICC/IF)

ARFIP2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

ARFIP2 Antibody (14548-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ARFIP2 Antibody in Immunocytochemistry (ICC/IF)

ARFIP2 Antibody in Immunocytochemistry (ICC/IF)

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARFIP2 Antibody in Western Blot (WB)

ARFIP2 Antibody in Western Blot (WB)

ARFIP2 Antibody in Western Blot (WB)

ARFIP2 Antibody in Western Blot (WB)

ARFIP2 Antibody in Western Blot (WB)

ARFIP2 Antibody in Western Blot (WB)

Product Image Gallery

ARFIP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ARFIP2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using ARFIP2 antibody 14548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver tissue slide using 14548-1-AP ( ARFIP2 Antibody) at dilution of 1:50 (under 40x lens).

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver tissue slide using 14548-1-AP ( ARFIP2 Antibody) at dilution of 1:50 (under 10x lens).

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

ARFIP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas tissue slide using 14548-1-AP (ARFIP2 Antibody) at dilution of 1:50.

ARFIP2 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ARFIP2 Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ARFIP2 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ARFIP2 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ARFIP2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ARFIP2 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 14548-1-AP (ARFIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

14548-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ARFIP2 Fusion Protein Ag6076 (138-341 aa encoded by BC000392)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: RKYESVLQL GRALTAHLYS LLQTQHALGD AFADLSQKSP ELQEEFGYNA ETQKLLCKNG ETLLGAVNFF VSSINTLVTK TMEDTLMTVK QYEAARLEYD AYRTDLEELS LGPRDAGTRG RLESAQATFQ AHRDKYEKLR GDVAIKLKFL EENKIKVMHK QLLLFHNAVS AYFAGNQKQL EQTLQQFNIK LRPPGAEKPS WLEEQ (138-341 aa encoded by BC000392)

Target Information

Plays a role in constitutive metalloproteinase (MMP) secretion from the trans Golgi network. May have important functions during vesicle biogenesis at certain cargo subdomains, which could be predominantly utilized by secreted MMPs, such as MMP7 and MMP2 (PubMed:26507660). Participates also in autophagy by regulating the starvation-depdendent trafficking of ATG9A vesicles which deliver the PI4-kinase to the autophagosome initiation site (PubMed:31204568). In addition, plays a role in NF-kappa-B inhibition by interacting with IKBKB and IKBKG (PubMed:26296658). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

ARFIP2 Regulates EMT and Autophagy in Hepatocellular Carcinoma in Part Through the PI3K/Akt Signalling Pathway.

Journal of hepatocellular carcinoma

Huang K,Lin Y,Wang K,Shen J,Wei D

Tue Jan 03 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: ADP-ribosylation factor interacting protein 2 (arfaptin 2); ADP-ribosylation factor-interacting protein 2; Arfaptin 2; Arfaptin-2; Partner of RAC1; partner of RAC1 (arfaptin 2); POR1

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Gene Aliases: 2310002N04Rik; ARFIP2; POR; POR1

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UniProt ID: (Human) P53365, (Rat) Q6AY65, (Mouse) Q8K221

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Entrez Gene ID: (Human) 23647, (Rat) 293344, (Mouse) 76932

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protein binding GTP binding protein domain specific binding GTP-dependent protein binding Rac GTPase binding phosphatidylinositol-4-phosphate binding cadherin binding involved in cell-cell adhesion

actin cytoskeleton organization movement of cell or subcellular component small GTPase mediated signal transduction lamellipodium assembly ruffle organization regulation of Arp2/3 complex-mediated actin nucleation cell-cell adhesion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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