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Invitrogen
ARHGAP21 Polyclonal Antibody
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FIGURE: 1 / 3
ARHGAP21 Antibody (PA5-100388) in ICC/IF
Immunofluorescent analysis of ARHGAP21 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ARHGAP21 polyclonal antibody (Product # PA5-100388) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100388
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ARHGAP21(Accession Q5T5U3), corresponding to amino acid residues A416-V466.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2849900
Product Specific Information
Antibody detects endogenous levels of total ARHGAP10.
Target Information
Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Rho GTPase-activating protein 10; Rho GTPase-activating protein 21; Rho-GTPase activating protein 10; Rho-type GTPase-activating protein 21
Gene Aliases: 5530401C11Rik; AA416458; ARHGAP10; ARHGAP21; KIAA1424
UniProt ID: (Human) Q5T5U3, (Mouse) Q6DFV3
Entrez Gene ID: (Human) 57584, (Mouse) 71435
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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