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ARHGAP22 Antibody (PA5-95800) in WB
Western blot analysis of ARHGAP22 in extracts of various cell lines. Samples were incubated with ARHGAP22 Polyclonal antibody (Product # PA5-95800) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-95800
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 510-650 of human ARHGAP22 (NP_0012429531)
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2807602
Product Specific Information
Immunogen sequence: NVPAPGLVPG IPSVASMAWS GASSSESSVG GSLSSCTACR ASDSSARSSL HTDWALEPSP LPSSSEDPKS LDLDHSMDEA GAGASNSEPS EPDSPTREHA RRSEALQGLV TELRAELCRQ RTEYERSVKR IEEGSADLRK R; Positive Samples: Mouse kidney, Mouse lung, Rat kidney, Rat lung; Cellular Location: Cytoplasm, Nucleus
Target Information
GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in down regulation of their active form. ARHGAP22 (Rho GTPase activating protein 22), also known as RHOGAP2, is a 698 amino acid protein that localizes to the cytoplasm, and contains one Rho-GAP domain and one PH domain. ARHGAP22 functions as a GTPase-activating protein for Rac 1 converting it to an inactive GDP-bound state and, through its interaction with ZNF161 (VEZF1), is thought to be useful in transcription regulation. Multiple isoforms of ARHGAP22 exist due to alternative splicing events.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: p68RacGAP; RHG22; Rho GTPase-activating protein 22; Rho-type GTPase-activating protein 22
Gene Aliases: Arhgap22; B230341L19Rik; p68RacGAP; RHOGAP2
UniProt ID: (Mouse) Q8BL80
Entrez Gene ID: (Mouse) 239027, (Rat) 306279
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