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Invitrogen
ARHGAP25 Polyclonal Antibody
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ARHGAP25 Antibody (PA5-89350) in WB
Western blot analysis of ARHGAP25 in extracts of various cell lines. Samples were incubated with ARHGAP25 Polyclonal antibody (Product # PA5-89350) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-89350
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_0011597481)
Conjugate
Form
Liquid
Concentration
0.42 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805511
Product Specific Information
Immunogen sequence: MSLGQSACLF LSIARSRSVM TGEQMAAFHP SSTPNPLERP IKMGWLKKQR SIVKNWQQRY FVLRAQQLYY YKDEEDTKPQ GCMYLPGCTI KEIATNPEEA GKFVFEIIPA SWDQNRMGQD SYVLMASSQA EMEEWVKFLR RVAGTPCGAV FGQRLDETVA YEQKFGPHLV PILVEKCAEF ILEHGRNEEG IFRLPGQDNL VKQLRDAFDA GERPSFDRDT DVHTVASLLK LYLRDLPEPV VPWSQYEGFL LCGQLTNADE AKAQQELMKQ LSILPRDNYS LLSYICRFLH EIQLNCAVNK MSVDNLATVI GVNLIRSKVE DPAVIMRGTP QIQRVMTMMI RDHEVLFPKS KDIPLSPPAQ KNDPKKAPVA; Positive Samples: Jurkat, A-549, Mouse lung, Rat spleen
Target Information
ARHGAP25 encode negative regulators of Rho GTPases, which are implicated in actin remodeling, cell polarity, and cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory protein Li 308; Rho GTPase-activating protein 25; Rho-type GTPase-activating protein 25
Gene Aliases: A130039I20Rik; ARHGAP25; HEL-S-308; KAIA0053; KIAA0053; RGD1562105
UniProt ID: (Human) P42331, (Mouse) Q8BYW1
Entrez Gene ID: (Human) 9938, (Rat) 500246, (Mouse) 232201
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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