Proteintech

ARHGEF1 Polyclonal Antibody

ARHGEF1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

ARHGEF1 Antibody (11363-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 11363-1-AP (ARHGEF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

ARHGEF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ARHGEF1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11363-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ARHGEF1 Fusion Protein Ag1905 (1-350 aa encoded by BC011726)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.39 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEDFARGAA SPGPSRPGLV PVSIIGAEDE DFENELETNS EEQNSQFQSL EQVKRRPAHL MALLQHVALQ FEPGPLVLRV PVPPNVAFEL DRTRADLISE DVQRRFVQEV VQSQQVAVGR QLEDFRSKRL MGMTPWEQEL AQLEAWVGRD RASYEARERH VAERLLMHLE EMQHTISTDE EKSAAVVNAI GLYMRHLGVR TKSGDKKSGR NFFRKKVMGN RRSDEPAKTK KGLSSILDAA RWNRGEPQVP DFRHLKAEVD AEKPGATDRK GGVGMPSRDR NIGAPGQDTP GVSLHPLSLD SPDREPGADA PLELGDSSPQ GPMSLESLAP PESTDEGAET ESPEPGDEGE P (1-350 aa encoded by BC011726)

Target Information

The Ras superfamily of GTPases, which can be subdivided into the Ras, Rho/Rac, Sar, Rab, ARF and Ran subfamilies, controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. The Ras superfamily of GTPases function as regulated switches that toggle between a biologically active GTP-bound and an inactive GDP-bound form. This activation is catalyzed by guanine nucleotide exchange factors (GEFs). The Dbl-related proteins are a large family of structurally related molecules that have a common ability to catalyze GEF activity for specific members of the Ras family. Dbl-related proteins include FGD1, Lsc, RhoGEF p115, Lfc, Lbc and Brx. Lsc, Lbc and Lfc share sequence homology and show exchange activity toward Rho family GTPases. RhoGEF p115 catalyzes GEF activity for Rho but not Rac, Cdc42 or Ras GTPases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression.

Nature metabolism

Qian L,Li N,Lu XC,Xu M,Liu Y,Li K,Zhang Y,Hu K,Qi YT,Yao J,Wu YL,Wen W,Huang S,Chen ZJ,Yin M,Lei QY

Sat Jul 01 00:00:00 UTC 2023

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Nature

Konermann S,Brigham MD,Trevino AE,Joung J,Abudayyeh OO,Barcena C,Hsu PD,Habib N,Gootenberg JS,Nishimasu H,Nureki O,Zhang F

Thu Jan 29 00:00:00 UTC 2015

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.

Nature

Konermann S,Brigham MD,Trevino AE,Joung J,Abudayyeh OO,Barcena C,Hsu PD,Habib N,Gootenberg JS,Nishimasu H,Nureki O,Zhang F

Thu Jan 29 00:00:00 UTC 2015

The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.

Molecular biology of the cell

Ryder PV,Vistein R,Gokhale A,Seaman MN,Puthenveedu MA,Faundez V

Mon Jul 01 00:00:00 UTC 2013

The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.

Molecular biology of the cell

Ryder PV,Vistein R,Gokhale A,Seaman MN,Puthenveedu MA,Faundez V

Mon Jul 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: 115 kDa guanine nucleotide exchange factor; 115-kD protein; Lbc's second cousin; Lsc homolog; lymphoid blast crisis like 2; Lymphoid blast crisis-like 2; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5

View more View less

Gene Aliases: ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5

View more View less

UniProt ID: (Human) Q92888, (Mouse) Q61210

View more View less

Entrez Gene ID: (Human) 9138, (Mouse) 16801

View more View less

Function(s)

Rho guanyl-nucleotide exchange factor activity GTPase activator activity protein binding poly(A) RNA binding guanyl-nucleotide exchange factor activity

Process(es)

Rho protein signal transduction cell proliferation regulation of Rho protein signal transduction positive regulation of GTPase activity negative regulation of axonogenesis termination of G-protein coupled receptor signaling pathway negative regulation of cell death

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

ARHGEF1 Polyclonal Antibody

ARHGEF1 Antibody (11363-1-AP) in ICC/IF

Product Details

11363-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!