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ARHGEF5 Antibody (A302-204A) in WB
Detection of human ARHGEF5 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ARHGEF5 antibody (Product # A302-204A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. ARHGEF5 was also immunoprecipitated by rabbit anti-ARHGEF5 antibody A302-203A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Product Details
A302-204A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 750 and 800 of human Rho guanine nucleotide exchange factor (GEF) 5.
Conjugate
Form
Liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
This gene encodes a protein that interacts with thyroid hormone receptors, also known as hormone-dependent transcription factors. The gene product interacts specifically with the ligand binding domain. This gene is one of several that may play a role in early-stage non-small cell lung cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARH5; DKFZp686N1969; Ephexin 3; Ephexin-3; Guanine nucleotide regulatory protein TIM; Oncogene TIM; p60 TIM; Rho guanine nucleotide exchange factor (GEF) 5; Rho guanine nucleotide exchange factor 5; Transforming immortalized mammary oncogene
Gene Aliases: ARHGEF5; GEF5; P60; TIM; TIM1
UniProt ID: (Human) Q12774
Entrez Gene ID: (Human) 7984
myeloid dendritic cell chemotaxis
actin cytoskeleton organization
regulation of actin cytoskeleton organization
regulation of Rho protein signal transduction
intracellular signal transduction
regulation of GTPase activity
positive regulation of JUN kinase activity
positive regulation of GTPase activity
positive regulation of sequence-specific DNA binding transcription factor activity
regulation of cytoskeleton organization
positive regulation of stress fiber assembly
regulation of ERK1 and ERK2 cascade
positive regulation of podosome assembly
positive regulation of protein import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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