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ARID1A Antibody (PA5-81489) in WB
Western Blot using ARID1A Polyclonal Antibody (Product # PA5-81489) at 1:500 dilution. Lane A: Rat liver tissue lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 242 kDa. Observed band size: 242 kDa.
Product Details
PA5-81489
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human ARID1A
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2788696
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human ARID1A.
Target Information
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARID domain-containing protein 1A; AT rich interactive domain 1A (SWI-like); AT-rich interactive domain-containing protein 1A; B120; BAF250; BAF250A; brain protein 120; BRG1-associated factor 250; BRG1-associated factor 250a; chromatin remodeling factor p250; h BM029; hELD; hOSA1; Osa homolog 1; OSA1 nuclear protein; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1
Gene Aliases: ARID1A; B120; BAF250; BAF250A; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1
UniProt ID: (Human) O14497
Entrez Gene ID: (Human) 8289
negative regulation of transcription from RNA polymerase II promoter
neural tube closure
cardiac chamber development
optic cup formation involved in camera-type eye development
nucleosome disassembly
chromatin remodeling
maintenance of chromatin silencing
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
covalent chromatin modification
intracellular estrogen receptor signaling pathway
androgen receptor signaling pathway
forebrain development
nucleosome mobilization
glucocorticoid receptor signaling pathway
ATP-dependent chromatin remodeling
positive regulation of transcription, DNA-templated
chromatin-mediated maintenance of transcription
cardiac muscle cell differentiation
placenta blood vessel development
toxin transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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