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Invitrogen
ARID5A Monoclonal Antibody (P18112)
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FIGURE: 1 / 5
ARID5A Antibody (MA5-18292) in IHC (F)
Immunohistochemistry (Frozen) analysis of ARID5A was performed in frozen-sectioned mouse mouse cerebellum tissue using ARID5A Monoclonal Antibody (P18112) (Product # MA5-18292) at a dilution of 1:250 (Green). Red: Calbindin, stained by Calbindin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Product Details
MA5-18292
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
P18112
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARID5A. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2539666
Product Specific Information
Recommended positive controls: 3xFlag-human ARID5A-transfected 293T, THP-1, THP-1 nuclear extract, Raw264.7, Raw264.7 (50 ng/mL LPS, 4hr).
Predicted reactivity: Mouse (83%), Bovine (88%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth (Patsialou et al., 2005 [PubMed 15640446]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARID domain-containing protein 5A; AT rich interactive domain 5A (Mrf1 like); AT rich interactive domain 5A (MRF1-like); AT-rich interactive domain-containing protein 5A; Modulator recognition factor 1; modulator recognition factor I; MRF-1; RFVG5814
Gene Aliases: ARID5A; D430024K22Rik; MRF-1; MRF1; RP11-363D14
UniProt ID: (Human) Q03989, (Mouse) Q3U108
Entrez Gene ID: (Human) 10865, (Mouse) 214855
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription, DNA-templated
chondrocyte differentiation
regulation of transcription, DNA-templated
positive regulation of histone acetylation
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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