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Invitrogen
ARK5 Polyclonal Antibody
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FIGURE: 1 / 1
ARK5 Antibody (PA5-110047) in WB
Western blot analysis of ARK5 in extracts of rat heart. Samples were incubated with ARK5 Polyclonal antibody (Product # PA5-110047) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-110047
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 362-661 of human NUAK1 (NP_055655.1).
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2855458
Product Specific Information
Immunogen sequence: LERQRSLKKS KKENDFAQSG QDAVPESPSK LSSKRPKGIL KKRSNSEHRS HSTGFIEGVV GPALPSTFKM EQDLCRTGVL LPSSPEAEVP GKLSPKQSAT MPKKGILKKT QQRESGYYSS PERSESSELL DSNDVMGSSI PSPSPPDPAR VTSHSLSCRR KGILKHSSKY SAGTMDPALV SPEMPTLESL SEPGVPAEGL SRSYSRPSSV ISDDSVLSSD SFDLLDLQEN RPARQRIRSC VSAENFLQIQ DFEGLQNRPR PQYLKRYRNR LADSSFSLLT DMDDVTQVYK QALEICSKLN
Target Information
NUAK1 (ARK5) is a serine/threonine kinase that plays a role in many cellular functions. The overexpression of this kinase has been associated with tumor invasion and metastasis (colorectal neoplasm). Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; Omphalocele kinase 1
Gene Aliases: ARK5; KIAA0537; NUAK1; OMPHK1; RGD1309956
UniProt ID: (Human) O60285
Entrez Gene ID: (Human) 9891, (Rat) 299694
protein phosphorylation
regulation of cell adhesion
regulation of myosin-light-chain-phosphatase activity
regulation of cellular senescence
cellular response to DNA damage stimulus
cell adhesion
intracellular signal transduction
regulation of cell proliferation
regulation of signal transduction by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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