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ARL2BP Antibody (MA5-29038) in ICC/IF
Immunofluorescence staining of ARL2BP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ARL2BP Recombinant Rabbit Monoclonal Antibody (1) (Product # MA5-29038, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).
Product Details
MA5-29038
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
001
Immunogen
Recombinant Human ARL2BP/BART1 protein (Met1-His163)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2784972
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human ARL2BP/BART1.
Target Information
ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2. GTP with high affinity but does not interact with ARL2. GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2. GTP but lack of ARL2 GTPase-activating protein activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADP-ribosylation factor like 2 binding protein; ADP-ribosylation factor like GTPase 2 binding protein; ADP-ribosylation factor-like 2 binding protein; ADP-ribosylation factor-like protein 2-binding protein; Arf-like 2 binding protein BART1; ARF-like 2-binding protein; ARL2-binding protein; Binder of ARF2 protein 1; binder of Arl Two; binder of Arl2; retinitis pigmentosa 66 (autosomal recessive)
Gene Aliases: ARL2BP; BART; BART1; RP66
UniProt ID: (Human) Q9Y2Y0
Entrez Gene ID: (Human) 23568
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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