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ARNT2 Antibody (PA5-101263) in ICC/IF
Immunocytochemistry analysis of ARNT2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ARNT2 polyclonal antibody (Product # PA5-101263) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101263
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ARNT2(Accession Q9HBZ2), corresponding to amino acid residues T30-T80.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850702
Product Specific Information
Antibody detects endogenous levels of total ARNT2.
Target Information
AhR, ARNT1, ARNT2 and BMAL1 are members of a family of transcription factors that contain a basic helix-loop-helix motif and a common "PAS" motif. The aromatic (aryl) hydrocarbon receptor, AhR, is a ligand dependent transcription factor that interacts with specific DNA sequences termed xenobiotic responsive elements (XREs) to activate several genes including CYP1A1, glutathione S-transferase Ya subunit and DT-diaphorase. The Ah receptor nuclear translocator proteins (Arnt 1 or Arnt 2) are required for ligand-dependent nuclear translocation of the Ah receptor and are also necessary for Ah receptor binding to the XRE element. BMAL1 (Brain and Muscle Arnt-like protein 1), also designated Arnt 3, TIC, JAP3 or MOP-3, has been shown to dimerize with Clock and bind to the promoter region of mPer1, suggesting that this protein plays a role in regulation of circadian oscillation in mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARNT 2; ARNT protein 2; Aryl hydrocarbon receptor nuclear translocator 2; aryl-hydrocarbon receptor nuclear translocator 2; bHLHe1; Class E basic helix-loop-helix protein 1
Gene Aliases: ARNT2; BHLHE1; Hif-2b; KIAA0307; mKIAA0307; WEDAS
UniProt ID: (Human) Q9HBZ2, (Mouse) Q61324, (Rat) Q78E60
Entrez Gene ID: (Human) 9915, (Mouse) 11864, (Rat) 25243
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
aryl hydrocarbon receptor binding
protein heterodimerization activity
DNA binding
signal transducer activity
protein dimerization activity
response to hypoxia
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
central nervous system development
brain development
positive regulation of cell proliferation
response to xenobiotic stimulus
response to toxic substance
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
in utero embryonic development
response to estradiol
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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