Proteintech

ASAH1 Polyclonal Antibody

ASAH1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

ASAH1 Antibody (11274-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 11274-1-AP (ASAH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ASAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASAH1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11274-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ASAH1 Fusion Protein Ag1799 (1-389 aa encoded by BC016828)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MNCCIGLGE KARGSHRASY PSLSALFTEA SILGFGSFAV KAQWTEDCRK STYPPSGPTV FPAVIRYRGA VPWYTINLDL PPYKRWHELM LDKAPVPGLL GNFPGPFEEE MKGIAAVTDI PLGEIISFNI FYELFTVCTS IVAEDKKGHL IHGRNMDFGV FLGWNINNDT WVITEQLKPL TVNLDFQRNN KTVFKASSFA GYVGMLTGFK PGLFSLTLNE RFSINGGYLG ILEWILGKKD AMWIGFLTRT VLENSTSYEE AKNLLTKTKI LAPAYFILGG NQSGEGCVIT RDRKESLDVY ELDAKQGRWY VVQTNYDRWK HPFFLDDRRT PAKMCLNRTS QENISFETMY DVLSTKPVLN KLTVYTTLID VTKGQFETYL RDCPDPCIGW (1-389 aa encoded by BC016828)

Target Information

ASAH1 encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. The processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene have been linked to the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. The related pathways include Innate Immune System and Sphingolipid metabolism. Diseases associated with ASAH1 include Farber Lipogranulomatosis and Spinal Muscular Atrophy With Progressive Myoclonic Epilepsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection.

Infection and drug resistance

Qi Q,Xu J,Wang Y,Zhang J,Gao M,Li Y,Dong L

Sun May 07 00:00:00 UTC 2023

Decreased Sphingosine Due to Down-Regulation of Acid Ceramidase Expression in Airway of Bronchiectasis Patients: A Potential Contributor to Pseudomonas aeruginosa Infection.

Infection and drug resistance

Qi Q,Xu J,Wang Y,Zhang J,Gao M,Li Y,Dong L

Sun May 07 00:00:00 UTC 2023

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis.

Nature communications

Boland S,Swarup S,Ambaw YA,Malia PC,Richards RC,Fischer AW,Singh S,Aggarwal G,Spina S,Nana AL,Grinberg LT,Seeley WW,Surma MA,Klose C,Paulo JA,Nguyen AD,Harper JW,Walther TC,Farese RV

Fri Oct 07 00:00:00 UTC 2022

Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Nature communications

Lang J,Bohn P,Bhat H,Jastrow H,Walkenfort B,Cansiz F,Fink J,Bauer M,Olszewski D,Ramos-Nascimento A,Duhan V,Friedrich SK,Becker KA,Krawczyk A,Edwards MJ,Burchert A,Huber M,Friebus-Kardash J,Göthert JR,Hardt C,Probst HC,Schumacher F,Köhrer K,Kleuser B,Babiychuk EB,Sodeik B,Seibel J,Greber UF,Lang PA,Gulbins E,Lang KS

Thu Mar 12 00:00:00 UTC 2020

Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.

International journal of molecular sciences

Su YT,Guo YB,Cheng YP,Zhang X,Xie XP,Chang YM,Bao JX

Fri Sep 20 00:00:00 UTC 2019

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Human molecular genetics

Kim MJ,Jeon S,Burbulla LF,Krainc D

Fri Jun 01 00:00:00 UTC 2018

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.

Cancer research

Tang YC,Yuwen H,Wang K,Bruno PM,Bullock K,Deik A,Santaguida S,Trakala M,Pfau SJ,Zhong N,Huang T,Wang L,Clish CB,Hemann MT,Amon A

Sun Oct 01 00:00:00 UTC 2017

Doxepin Mitigates Noise-induced Neuronal Damage in Primary Auditory Cortex of Mice via Suppression of Acid Sphingomyelinase/Ceramide Pathway.

Anatomical record (Hoboken, N.J. : 2007)

Su YT,Meng XX,Zhang X,Guo YB,Zhang HJ,Cheng YP,Xie XP,Chang YM,Bao JX

Fri Dec 01 00:00:00 UTC 2017

Doxepin Mitigates Noise-induced Neuronal Damage in Primary Auditory Cortex of Mice via Suppression of Acid Sphingomyelinase/Ceramide Pathway.

Anatomical record (Hoboken, N.J. : 2007)

Su YT,Meng XX,Zhang X,Guo YB,Zhang HJ,Cheng YP,Xie XP,Chang YM,Bao JX

Fri Dec 01 00:00:00 UTC 2017

Doxepin Mitigates Noise-induced Neuronal Damage in Primary Auditory Cortex of Mice via Suppression of Acid Sphingomyelinase/Ceramide Pathway.

Anatomical record (Hoboken, N.J. : 2007)

Su YT,Meng XX,Zhang X,Guo YB,Zhang HJ,Cheng YP,Xie XP,Chang YM,Bao JX

Fri Dec 01 00:00:00 UTC 2017

Doxepin Mitigates Noise-induced Neuronal Damage in Primary Auditory Cortex of Mice via Suppression of Acid Sphingomyelinase/Ceramide Pathway.

Anatomical record (Hoboken, N.J. : 2007)

Su YT,Meng XX,Zhang X,Guo YB,Zhang HJ,Cheng YP,Xie XP,Chang YM,Bao JX

Fri Dec 01 00:00:00 UTC 2017

Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.

Journal of dermatological science

Sawada E,Yoshida N,Sugiura A,Imokawa G

Mon Oct 01 00:00:00 UTC 2012

Journal of dermatological science

Sawada E,Yoshida N,Sugiura A,Imokawa G

Mon Oct 01 00:00:00 UTC 2012

A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.

Archives of dermatological research

Yoshida N,Sawada E,Imokawa G

Sat Sep 01 00:00:00 UTC 2012

A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.

Archives of dermatological research

Yoshida N,Sawada E,Imokawa G

Sat Sep 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: AC; ACDase; acid CDase; Acid ceramidase; Acylsphingosine deacylase; Glycosylceramide deacylase; N-acylethanolamine hydrolase ASAH1; N-acylsphingosine amidohydrolase; N-acylsphingosine amidohydrolase (acid ceramidase) 1; N-acylsphingosine amidohydrolase 1; PHP32; Putative 32 kDa heart protein

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Gene Aliases: 2310081N20Rik; AC; ACDase; ASAH; ASAH1; HSD-33; HSD33; PHP; PHP32; SMAPME

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UniProt ID: (Human) Q13510, (Mouse) Q9WV54, (Rat) Q6P7S1

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Entrez Gene ID: (Human) 427, (Mouse) 11886, (Rat) 84431

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Function(s)

catalytic activity ceramidase activity hydrolase activity molecular_function

Process(es)

lipid metabolic process response to organic substance lung development ceramide metabolic process glycosphingolipid metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ASAH1 Polyclonal Antibody

ASAH1 Antibody (11274-1-AP) in ICC/IF

Product Details

11274-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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