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Invitrogen
ASAH3 Polyclonal Antibody
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ASAH3 Antibody (PA5-103592) in IHC (P)
Immunohistochemistry analysis of ASAH3 in mouse testis tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with ASAH3 polyclonal antibody (Product # PA5-103592) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103592
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ACER1(Accession Q8TDN7), corresponding to amino acid residues P139-S189.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852928
Product Specific Information
Antibody detects endogenous levels of total ASAH3.
Target Information
Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.5.1.23; Acylsphingosine deacylase 3; alkaline CDase 1; Alkaline ceramidase 1; AlkCDase 1; CTB-180A7.3; maCER1; N-acylsphingosine amidohydrolase (alkaline ceramidase) 3; N-acylsphingosine amidohydrolase 3
Gene Aliases: 2310024P18Rik; ACER1; AI662009; ALKCDase1; ASAH3; Cer1
UniProt ID: (Human) Q8TDN7, (Mouse) Q8R4X1
Entrez Gene ID: (Human) 125981, (Rat) 301118, (Mouse) 171168
sphingolipid metabolic process
epidermis development
response to alkaline pH
regulation of lipid metabolic process
sphingolipid biosynthetic process
cell differentiation
keratinocyte differentiation
sphingosine biosynthetic process
ceramide catabolic process
cellular response to calcium ion
lipid metabolic process
ceramide metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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