Invitrogen

ASK1 Monoclonal Antibody (2E4)

ASK1 Antibody in Immunocytochemistry (ICC/IF)

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ASK1 Antibody (MA5-15861) in ICC/IF

Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody (Product # MA5-15861) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

ASK1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

ASK1 Antibody — Sdc1-coupled IGF1R sequesters and inactivates ASK1 A. ASK1 immunoprecipitates from CAG or MM.1R cells treated for 6 hr with 10 µM SSTNIGF1R or 10 µM IGF1R inhibitor AG538 are probed for inactive (pY, pSer83 and pSer966), active (pThr838) or total ASK1; B. Sdc1 immunoprecipitates from CAG or MM.1R cells treated with 10 µM SSTNIGF1R or 10 µM AG538 are probed for the presence of total ASK1, inactive (pY) or active (pThr838) ASK1, active IGF1R (pY1131) or total IGF1R, and total Sdc1 (mAb B-A38); C. RNAi knock-down of ASK1 and IGF1R protein levels in CAG and MM.1R cells 48 hr after control (Ctrl) or target-specific siRNA transfection; D. Equal cell equivalents from control, ASK1 or IGF1R siRNA-transfected CAG and MM.1R cells (48 hr post-transfection) treated with or without 30 µM SSTNIGF1R for 8 hr are analyzed for levels of active JNK (pThr183/pTyr185) and total JNK; E. Active Caspase 3/7 is quantified in control, IGF1R or ASK1 siRNA-transfected CAG and MM.1R cells treated as in (D). {KD}

Product Details

MA5-15861

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E4

Immunogen

Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11152150

Product Specific Information

MA5-15861 targets MAP3K5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15861 immunogen is purified recombinant fragment of human MAP3K5 expressed in E. Coli. .

MA5-15861 detects MAP3K5 which has a predicted molecular weight of approximately 55kDa.

Target Information

MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prdx1 Interacts with ASK1 upon Exposure to H₂O₂ and Independently of a Scaffolding Protein.

Antioxidants (Basel, Switzerland)

Vo TN,Malo Pueyo J,Wahni K,Ezeriņa D,Bolduc J,Messens J

MA5-15861 was used in Western Blot to reveal that cytosolic Prdx redox-relays can be organized in different ways and yet again highlight the differentiated roles of Prdx1 and Prdx2.

Wed Jun 30 00:00:00 UTC 2021

Syndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth.

Cancer research

Beauvais DM,Jung O,Yang Y,Sanderson RD,Rapraeger AC

MA5-15861 was used in western blot to elucidate how syndecan-1 contributes to multiple myeloma

Thu Sep 01 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: apoptosis signal regulating kinase 1; Apoptosis signal-regulating kinase 1; ASK 1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Mitogen-activated protein kinase kinase kinase 5; RP3-325F22.4

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Gene Aliases: ASK1; MAP3K5; MAPKKK5; MEKK5

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UniProt ID: (Human) Q99683

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Entrez Gene ID: (Human) 4217

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Function(s)

magnesium ion binding protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity protein binding ATP binding protein kinase binding protein phosphatase binding protein homodimerization activity

Process(es)

MAPK cascade activation of MAPKK activity response to ischemia protein phosphorylation JNK cascade activation of JUN kinase activity intrinsic apoptotic signaling pathway in response to oxidative stress viral process response to endoplasmic reticulum stress p38MAPK cascade positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of JUN kinase activity innate immune response positive regulation of myoblast differentiation positive regulation of JNK cascade intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide apoptotic signaling pathway programmed necrotic cell death positive regulation of neuron death cellular response to reactive nitrogen species

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ASK1 Monoclonal Antibody (2E4)

ASK1 Antibody (MA5-15861) in ICC/IF

Product Details

MA5-15861

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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